fig5

Figure 5. A549BTZR TRAIL-induced apoptosis was mediated via TRAIL-R1, which was redistributed into lipid rafts. (A) Cell death was determined in the A549 and A549BTZR cells after exposure to 100 ng/mL TRAIL, 50 ng/mL 4C7 (TRAIL-R1-specific TRAIL variant), or 50 ng/mL D269HE195R (TRAIL-R2-specific TRAIL variant) for 24 h. The effect of TRAIL and TRAIL-R1 was significant in A549BTZR cells compared to A549 parental (P < 0.0001); (B) Western blot analysis of the TRAIL-R1 and -R2 expression levels in different cellular fractions, with fractions 5-7 and fractions 8-10 representing the lipid and non-lipid rafts, respectively. The values are the means ± SEM of at least three independent experiments, while blots are also representative of at least 3 separate experiments. TRAIL: TNF-related apoptosis-inducing ligand; TNF: tumor necrosis factor.