fig7
Figure 7. Effect of simvastatin and CHR2863 combinations on Rheb prenylation. U937/WT, U937/CHR2863R0.2 and U937/CHR2863R5 cells were incubated for 48 h with simvastatin, CHR2863, and their combination (as described in Figure 5), with or without the addition of MVA (100 µM), FPP (2 µM), GGPP (1 µM) or FTI-277 (10 µM). The slower (upper) migrating band represents unprenylated Rheb, and the faster (lower) migrating band represents prenylated Rheb. CHR2863: (6S)-[(R)-2-((S)-Hydroxy-hydroxycarbamoyl-methoxy-methyl)-4-methyl-pentanoylamino]-3,3 dimethyl-butyric acid cyclopentyl ester.
