fig5
From: Circular RNA circNCOA3 promotes tumor progression and anti-PD-1 resistance in colorectal cancer
Figure 5. CircNCOA3 functions as a molecular sponge for miR-203a-3p.1. (A) RIP assays in CRC cells stably expressing Flag-AGO2 or Flag-GFP; (B) Luciferase activity of the circNCOA3 reporter in HCT116 cells transfected with circNCOA3 shRNA; (C) Luciferase activity of circNCOA3 reporter in CRC cells transfected with various miRNAs (miR-203a-3p.1, miR-217, miR-222); (D) RNA pulldown using a biotin-labeled circNCOA3 probe in CRC cells; (E) RNA pulldown using a biotin-labeled circNCOA3 probe in CRC cells transfected with sh-circNCOA3; (F) Correlation between the expression of circNCOA3 and miR-203a-3p.1 in 78 CRC tissues. RIP: RNA immunoprecipitation; CRC: colorectal cancer.
