fig1

Figure 1. Characteristics of the obtained subline MCF7/HT. (A) - the viability of MCF7/HT and MCF7 cells in the presence of 4-hydroxytamoxifen (HT), *P < 0.05; (B) - Representative immunoblotting images for sensitive MCF7 and resistant MCF/HT cells with antibodies against ERα, p-Akt, and Akt; the cells were treated with 5 µM HT for 24 h and then subjected to immunoblot analysis; (C) - Densitometry for immunoblotting data (n = 3) was performed using ImageJ software (Wayne Rasband, NIH). The protocol for analysis was provided by The University of Queensland with the recommendations from the work^[51]; *P < 0.05 vs. corresponding control cells; ^#P < 0.05 vs. MCF7 cells.