fig2

Figure 2. Gene Expression Levels for Tyrosine Kinases in Leukemic Cells from Pediatric Patients with KMT2A/MLL-R⁺ B-ALL vs. Other Types of B-ALL without KMT2A/MLL Rearrangements. Probeset level normalized signal values from the archived data sets GSE11877 and GSE13351 were examined in these comparisons. The cluster figure displays the expression levels in KMT2A/MLL-R⁺ ALL cells mean centered to the reference group (other subsets of ALL without KMT2A/MLL rearrangements) represented by log₂-transformed fold change values (blue to red color indicates under-expression to over-expression respectively in KMT2A/MLL-R⁺ samples). Co-regulated probesets are organized and depicted by dendrograms for both probesets (rows) and patients (columns). Depicted are the differential gene expression changes of log₂-transformed, robust multi-array analysis (RMA) normalized values for 25 pediatric ALL KMT2A/MLL-R⁺ cases (GSE11877 and GSE13351) compared to 94 non-MLL-R⁺ other samples (GSE11877 and GSE13351) exhibiting 20 dysregulated probesets, of which 14 were upregulated in KMT2A/MLL-R⁺ subset of cases. FLT3_206674_at was the most significantly upregulated probeset (Fold Change = 8.65; P-value < 10^-8) followed by BLK_206255_at (Fold Change = 3.11; P-value < 10^-8) and HCK_208018_s_at (Fold Change = 2.64; P-value < 10^-8) [Supplementary Table 5].