fig3

Figure 3. Butein-instigated increase of miR-186-5p affected the sphere forming ability and the cisplatin sensitivity of 3D-grown NCI-H2373 cells. (A) Spheroid formation assay: NCI-H2373 cells, treated with ctrl-(DMSO 0.01%) (a, b) or butein (10 µM, 8 h) (c, d), transfected with miR-186-5p mimic (e, f), or with PCDNA3-TWIST1(g, h), were clonally seeded and allowed to form spheroids for 48 h. After that, ctrl (saline: a, c, e, g) or cisplatin (b, d, f, h) was added at the IC₂₅. Representative micrographs of the formed spheroids (on Day 2 after cisplatin or saline treatment). Scale bar, 200 µm. (B) Histograms showing quantitation of the SFE from NCI-H2373 cells treated with saline or cisplatin at IC₂₅ and IC₅₀, respectively, and counted on Day 7 after treatment started. The percentage is relative to the control sample within the group except when otherwise indicated. Asterisk indicate statistical significance as follows: *P < 0.05; **P < 0.01; ns: not significant (P > 0.05). The average of 3 experiments is shown.