fig5

Figure 5. Effect of GW4869 treatment on paclitaxel-resistant DU145-R cells. EVs were isolated from the conditioned media of paclitaxel-resistant DU145-R cells following 24 h (upper panel) and 48 h (lower panel) of treatment with GW4869 (10-20 µM) and characterized for concentration and size distribution by NTA. (A-B) Each colored line in the left panel represents the mean of three samples, and an average data of 5 videos of 30 s each was used for each sample. Concentration (particles/mL)-size distribution, average EV concentration per million cells, average size, and percent particles for various size ranges are presented. Each bar represents mean ± SEM (n = 3). *P < 0.05, **P < 0.005 ***P < 0.0005 ****P < 0.0001. (C) Colony formation was measured in DU145-R cells after GW4869 treatment (10 and 20 μM) as described in the methods. Representative images are shown (left panel), and the colony number is presented as mean ± SEM (n = 3 replicates per group). ***P < 0.0005, ****P < 0.0001.