fig2

Figure 2. Effect of GW4869 treatment on the survival and clonogenicity of PC-R cells. (A) Paclitaxel-resistant PC3-R cells were treated with DMSO (VC) or GW4869 (5-20 µM), and cell viability was measured in MTT assay after 24, 48, and 72 h. Data are presented as mean ± SEM (n = 5 replicates per group). *P < 0.05, **P < 0.005. (B) Colony formation was measured in PC3-R cells after GW4869 treatment (10 and 20 μM) as described in the methods. Representative images are shown (left panel), and the colony number is presented as mean ± SEM (n = 3 replicates per group). ****P < 0.0001. VC: Vehicle control.