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Figure 1. Characterization of EV concentration and size distribution following GW4869 treatment in PC3-R cells. EVs were isolated from the conditioned media of paclitaxel-resistant PC3-R cells following 24 h (upper panel) and 48 h (lower panel) of treatment with GW4869 (10-20 µM) and characterized for concentration and size distribution by NTA. (A) Concentration and size distribution of EVs isolated from DMSO (VC) or GW4869 (10 and 20 µM) treated cells are represented with green, orange, and red colors, respectively (n = 3). Each line represents the mean of three samples, and an average data of 5 videos of 30 sec each was used for each sample. (B-C) Total EV concentration per million cells and mean size are plotted. (D) Size distribution of EVs is presented as percent particles in the mentioned size range. Each bar represents mean ± SEM (n = 3). VC: Vehicle control; *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.0001.