fig4

Figure 4. A: Shows MCF10A cells stably expressing the indicated genes were grown in 3D Matrigel in EGF/insulin-free medium + 1% charcoal/dextran-stripped serum (CSS) treated with vehicle [dimethyl sulfoxide (DMSO)], 20 nM neratinib, 1 mM alpelisib, or the combination. Scale bars, 250 µm. B: Shows the number of colonies with invasive branching per field of view (FOV) from (B) was quantified. Data represent the average ± SD (n = 3). Figure and legend used from Hanker et al.^[23].