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Figure 5. Combination of SMI and cisplatin induced apoptosis in human lung adenocarcinoma A549/DDP cells. (A) Apoptosis detection by Hoechst staining. White arrows show apoptotic cells with nuclear condensation (400×). (B) Cells were dyed by Annexin V-FITC/PI and detected by flow cytometry. (C) Simple Western blot detection for apoptosis-related proteins (Bcl-2, Bax, and cleaved-caspase 3). SMI: Shenmai injection.