fig4

Figure 4. Antiproliferative activity and induction of cell death by IS1 and IS4 in PDAC cells. (A) Representative growth inhibition curves of PDAC cells Hs766t treated for 72 h with the compounds IS1, IS2, IS3 and IS4. Points, mean values; bars, Standard Error of the Mean (SEM). (B) Table summarising the IC₅₀ values of the IS1, IS2, IS3 and IS4 compounds against the PDAC cells SUIT-2 and Hs766t. The values are reported as means ± SEM of three separate experiments. IS1 and IS4 had the lowest IC₅₀ values and statistical analyses showed significant differences of these compounds compared to both IS2 and IS3. Thus, we selected these compounds for the following studies. (C) Representative bar graph of trypan blue exclusion assay, showing the percentage of viable Panc05.04 cells after treatment for 96 h and the compound IS1 and IS4 at five different concentrations (0.1, 0.5, 1, 5 and 10 µM). Columns, mean values; bars, SEM.