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Figure 3. Oral administration of BJ reduced xenograft spheroid tumor growth. (A) BJ extract suppressed the growth of xenograft tumors. Nude mice with established xenograft spheroid tumors of H1975 cells as described in “Methods” were administered orally with 2 and 4 g of BJ/kg daily for 6 consecutive days (arrow). Each group contained four mice. The graph represents tumor growth variation (y-axis) from the start of feeding (x-axis). **P < 0.01 indicates a significant difference in the measured tumor volumes between mice fed with 2 and 4 g BJ/kg and those with water from three individual experiments with four mice in each group. (B) The bodyweight of nude mice. No significant differences of the average body weight in mice with established xenograft tumors orally administered with water and BJ extract daily for 6 consecutive days (arrow) were found. The graph represents variations of mice weight (y-axis) from the start of feeding in days (x-axis). (C) Resected tumor weight reduction. Tumor weights of the xenograft tumors were measured in mice with gavage feeding of BJ in comparison with those of water. The horizontal bars represent mean values of tumor mass as collected following different treatments. **P < 0.01 indicates significant weight difference between mice fed with 2 and 4 g of BJ/kg and those with water. The graph is representative of three independent experiments. (D) Hematoxylin and eosin (HE) staining. The paraffin-embedded spheroid tumors as treated with BJ (2 and 4 g/kg) and water-fed mice control were dissected, stained with HE, and analyzed by confocal microscopy (scale bar, 25 μm). The white arrow signifies apoptotic body location.