fig3

Figure 3. (A) The monolayer culture did not have significantly different 340/380 ratios among the groups. (B) In the spheroid culture, the combination treatment group showed a significantly reduced 340/380 ratio compared to the negative control, where 380 nm indicates the intensity of the Fura-2 dye when not bound to calcium and 340 nm indicates the intensity of the Fura-2 dye intensity when bound to calcium. (C) The Fura-2 dye presented high intensity at the periphery of the spheroid, which is a proliferating region of a spheroid. At the same time, the dye intensity in the core was low. *P < 0.05. Scale bar: 200 μm. PTX: Paclitaxel.