fig6

Figure 6. Panobinostat plus pharmacological HDAC6 inhibitors induced cell death synergistically. (A) RMPI-8226 and Kas6 were treated with various concentrations of the HDAC6-specific inhibitors, ricolinostat, or tubaicin for 48 h, and cell viability was measured. Viability was normalized to control treated with vehicle. (B) RPMI8226wt and Kas6wt were treated with single agent ricolinostat, tubaicin or panobinostat; or a combination of ricolinostat plus panobinostat or tubacin plus panobinostat for 48 h. Cell viability was measured after treatment using trypan blue assay, and combination index was calculated by Isobologram analysis. HDAC: Histone deacetylase.