fig6
Figure 6. Hsa_circ_0020714 promotes non-small cell lung cancer (NSCLC) resistance to resistance to anti-PD-1 immunotherapy in NSCLC patients. (A) Putative miR-30a-5p binding sites to mouse Sox4 were predicated via StarBase v3.0. (B) The luciferase activity of wild type (wt) or mutant (mu) pLG3-Sox4 mRNA 3′-UTR in the HEK-293T cells after cotransfected with miR-30a-5p. (C) Sox4 expression in the wild type or mutant hsa_circ_0020714-overexpressing cells was detected by western blot. (D) LLC cells with mutant or wild type hsa_circ_0020714-overexpressing were subcutaneously injected into 4-week-old C57BL/6 mice. When tumors had reached a mean volume of 100 mm3, the mice were treated with an IgG or PD-1 antibody. The data are presented as the mean tumor volume (n = 6). (E) The data are expressed as the percentage of tumor growth inhibition (the data are presented as the mean ± SD). (F) The survival curves of the mouse lung xenograft tumors formed by mutant or wild type hsa_circ_0020714-overexpressing cells and treated with a mouse antibody against mouse PD-1. ***P < 0.001, *P < 0.05. NS: Not significant.
