fig3

Figure 3. Hsa_circ_0020714 upregulates SOX4 expression via sponging miR-30a-5p in NSCLC cells. (A) Putative miR-30a-5p binding sites to hsa_circ_0020714 and SOX4 were predicated via StarBase v3.0. (B) Hsa_circ_0020714 expression was detected in six NSCLC cell lines using qRT-PCR. (C) CircRIP analysis were carried out using an AGO2 antibody in A549 cells extract. (D) The luciferase activity of wild type (wt) or mutant (mu) pLG3-hsa_circ_0020714/SOX4 mRNA 3′-UTR in the HEK-293T cells after cotransfected with miR-30a-5p. (E) Hsa_circ_0020714 expression was modified in the NCI-H460 cells by lentivirus-mediated cDNA transfection. (F, G) SOX4 mRNA and protein expression levels in wild type (wt) or mutant (mu) hsa_circ_0020714-overexpressing NSCLC cells. ***P < 0.001, **P < 0.01, *P < 0.05. NS: Not significant.