fig7

Figure 7. In vitro cytotoxicity of PLAD-MLP and comparators on the multidrug-resistant, NCI/ADR tumor cell line. Identical experiment as described in Figure 6, except for the use of the NCI/ADR human cell line. In this experiment, we used PL-MLP loaded with Ald (=PLA-MLP) as control to rule out any contribution of Ald to the enhanced cytotoxic effect of PLAD-MLP. Note the greater cytotoxicity of MMC as compared to Dox, as expected from the MDR-1, P-glycoprotein mediated mechanism of resistance which spares MMC. Note, as well, the greater cytotoxicity of PLAD-MLP over a combination of PLA-MLP and PLD. PLAD-MLP values were significantly different from other liposome formulations (paired t test, P < 0.05)