fig6

Figure 6. In vitro cytotoxicity of PLAD-MLP and comparators. Tumor cells were seeded in 96-multiwell plates. After overnight incubation, the liposomal drugs were added in triplicates for each concentration and cells were incubated further for 72 h. Cell growth was evaluated colorimetrically with methylene blue staining and growth rates were calculated as previously described^[25]. Upper and lower panels show results for Dox and MLP/MMC cytotoxicity in T24 human bladder tumor cells and HT29 human colon tumor cells, respectively. Note the greater cytotoxicity of the multidrug formulation, PLAD-MLP (red lines), over the combined addition of PL-MLP and PLD at equal drug concentrations. PLAD-MLP values were significantly different from other liposome formulations (paired t test, P < 0.05)