fig2

Figure 2. All gemR models showed increased levels of proliferation marker Ki67. Tumor tissue harvested on days 190, 120, and 179 for PA4.gemR, PA10.gemR and PA16.gemR, respectively, was stained with H&E to visualize tumor and stroma morphology, and was immunostained for the proliferation marker Ki67. Tumor tissue harvested from control mice bearing PA4, PA10 or PA16 tumors (gemcitabine-naïve) served as controls for IHC staining experiments. Scale bar = 10 μm (A); data in ‘A’ were quantitated to determine Ki67 proliferation indices (%). Bar graphs show mean ± S.E.M. Ki67 expression. Data were analyzed using Student’s t-test (***P < 0.001, ****P < 0.0001) (B). gemR: gemcitabine resistance; IHC: immunohistochemistry