fig1

Figure 1. DCA enhances the antitumor effect of THP in liver cancer cells. A: Hep3B and Huh7 cells were treated with THP at different concentrations (0, 150, 300, 600 or 900 nmol/L) for 24 h, and cell viability was then determined with a CCK-8 kit; B: Hep3B and Huh7 cells were treated with DCA at different concentrations (0, 10, 20, 40 or 60 mmol/L) for 24 h, and cell viability was then determined with a CCK-8 kit. The experiment is representative of at least three independent experiments. ^**P < 0.01; C, D: the liver cancer cell lines Hep3B and Huh7 were treated with DCA (20 mmol/L), THP (300 nmol/L) or a combination of DCA and THP for 24 h. Cell morphology was then observed under a microscope (C) and cell viability was determined with a CCK-8 kit (D). Experiments were repeated at least three times. ^**P < 0.01. DCA: dichloroacetate; THP: pirarubicin