fig3

Figure 3. Photokilling of glioblastoma cells: inhibitory effects of nitric oxide from stress-upregulated inducible NO synthase (iNOS). (A) aminolevulinic acid (ALA)-treated U87 cells were irradiated with broad-band visible light in the absence (○) vs. presence of 1400W (□) or cPTIO (Δ). A light-only or ALA-only control was run alongside (×); (B) U87 apoptosis after ALA/light treatment: stimulation by 1400W (W) or L-NAME (N). Values are relative to a camptothecin (CPT) standard; (C) immunoblot of iNOS and nNOS in photostressed U87 cells; (D) immunoblot of iNOS in photostressed U251 cells. (D,C): DC represents ALA-only dark control. Numbers below bands indicate NOS band intensity relative to β-actin and normalized to DC^[42]