fig2

Figure 2. Immunohistochemical analysis of TMA in adjacent normal prostate tissue and adenocarcinoma (stage IV). Immunohistochemical staining was performed with an antibody to CD44-ICD in prostate cancer tissue array with adjacent normal prostate tissue. Sections were then scanned using an Aperio Scanscope^® CS instrument (Aperio Scanscope CS system, Vista, CA, USA). A, B: represent normal prostatic and adenocarcinoma (stage IV) tissue sections, respectively. These sections are magnified in A’, A’’, B’ and B’’. Staining was repeated two times. Scale bar represents 500 µm (A and B), 100 µm (A’ and B’), and 25 µm (A” and B”); C, D: the protein expression pattern is expressed as percent cells stained per core for CD44-ICD and CD44s proteins and presented as a graph. Data are given as a scatterplot for the indicated number of cores analyzed in Table 2. The number of cores that were analyzed by two investigators are provided in the parentheses of the first column denoted as “Grade” in Table 2. CD44: Cluster of differentiation 44; ICD: intracellular domain