fig1

From: Entrapment of DNA topoisomerase-DNA complexes by nucleotide/nucleoside analogs

Figure 1. Substitution of nucleoside analogs into a model Top1 cleavage site results in Top1cc. (Top) The chemical structures for three nucleoside analogs that induce Top1cc in genomic DNA; (Middle) sequence of a model Top1cc used to investigate nucleoside analog substitution effects on Top1cc with the +1 site in the non-scissile strand highlighted; and (Bottom) 23mer model Top1cc substrate used to study cleavage/re-ligation effects of nucleoside analogs. *A corresponds to the [³²P]-cordycepin label. Modified from References^[27,39,46]. Top1: topoisomerase 1; AraC: cytarabine; GEM: gemcitabine; FdU: 5-fluoro-2’-deoxyuridine; Top1cc: Top1 cleavage complex