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Figure 4. Autoradiogram of denaturing sequencing gel (Taq polymerase stop assay) showing alkylation sites induced by N3P and its ring-opened triazene (N3P tz). Control lane, unmodified BamHI fragment of pBR322 DNA; cisplatin (1 μm); Temozolomide (TMZ) (1000 μm); N3P (1 μm, 10 μm, 100 μm, 1000 μm); N3P tz (1 μm, 10 μm, 100 μm, 1000 μm). Arrows, indicate positions and sequence of binding sites on plasmid DNA. Drug-DNA incubations were for 2 h at 37 °C
