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Figure 2. Expression levels of ABCB1 in breast cancer cell lines (MCF-7 and KCR). ABCB1 mRNA levels were analysed by RT-qPCR and the average fold change of ABCB1 gene expression was calculated using 2^-(ΔΔCt) method (A). ABCB1 was identified by immunoblotting using β-actin as an internal control (B) Localization of ABCB1 expression was performed in cells incubated with anti-human ABCB1-FITC-conjugated and counter-stained with DAPI. Cells were observed by fluorescence microscopy and images were acquired (C). DNA nuclear - blue; ABCB1 - green. **** P < 0.001