fig3

Figure 3. Representative images of mCRPC patient CTCs with quantitation of CRLX301-induced MT-DTE. A: single CTCs from patients 1-3 at different time points as indicated. CTCs are stained for tubulin (magenta), Pan-CK (red) and DAPI (blue) and imaged by high-resolution confocal microscopy. Maximum intensity projections are shown. Scale bar = 5 μM; B: graphical representation of mean MT-DTE (displayed as Mean ± SEM) at C1D1, C1D8, C2D1 and C2D8. mCRPC: metastatic castration-resistant prostate cancer; CTCs: circulating tumor cells; MT-DTE: microtubule drug-target engagement; CK: cytokeratin; DAPI: 4′, 6-diamidino-2-phenylindole