fig2

Figure 2. Workflow of quantitation of MT-DTE in representative images of mCRPC patient CTCs. Representative CTCs with different levels of DTE are shown, from least engaged (top row) to most engaged (bottom row). We quantified drug-induced MT-DTE using ImageJ as described in Methods. Tubulin maximum intensity projection is shown in column A, converted to the 8-bit TIFF image (column B). Column C shows visually the number of pixels corresponding to the top 25% of fluorescence intensity in each cell. Table shows resulting integrated density values (MT-DTE) for each single CTC. Scale bar = 20 μM. DTE: drug-target engagement; MT-DTE: microtubule drug-target engagement; mCRPC: metastatic castration-resistant prostate cancer; CTCs: circulating tumor cells