fig7

Figure 7. MAPK signaling and STAT activation are inversely correlated in human TNBC cell lines and basal-like patient samples. A: immunoblot analysis of HCC1143, HCC1954, and MDA-MB-231 cells grown in either base media or PMEC media treated with or without trametinib (50 nM; MEKi) for 48 h prior to lysis; B: immunoblot analysis of HCC1143 cells grown in RPMI media supplemented with EGF (20 ng/mL), hydrocortisone (0.5 µg/mL), or insulin (10 µg/mL) along with being treated with or without trametinib (50 nM; MEKi) for 48 h prior to lysis; C: transcriptional analysis of STAT1 and Ras/MAPK scores for 211 Basal-like breast cancers. MAPK: mitogen activated protein kinase; TNBC: triple negative breast cancer; MEKi: MEK inhibition