fig4

Figure 4. Potential involvement of Ca²⁺/Mg²⁺-dependent endonuclease in DNA fragmentation. A: We observed no obvious reduction in full-length ICAD levels during apoptosis of miPS-LLCcm. miPS-LLCcm cells were treated with 100 nmol/L of daunorubicin for the indicated periods and analyzed by western blotting analyses; B: detection of processed caspases in attached or floating cells; C: apoptotic cells in the presence of BAPTA-AM were detected by annexin V staining. Cells were pretreated with 10 μmol/L of BAPTA-AM for 1 h, treated with 100 nmol/L daunorubicin, and analyzed by FACS; D: Ca²⁺ chelator did not affect caspase levels during apoptosis of miPS-LLCcm cells; E: suppression of DNA fragmentation by treatment with a Ca²⁺ chelator. DNA fragmentation in the cells treated with daunorubicin in the presence of 10 μmol/L of BAPTA-AM was analyzed