fig7

Figure 7. Augmented expression of WT1 in relapsed pediatric B-lineage acute lymphoblastic leukemia (ALL). Gene expression profiling data on primary leukemia cells from relapsed (n = 76; combined datasets from GSE18497 and GSE28460) vs. newly diagnosed (n = 474) B-lineage ALL patients were compared. Mixed ANOVA model was utilized to determine significant differences in expression between new diagnosis and relapse cases. A: A two-way agglomerative hierarchical clustering technique was used to organize expression patterns using the average distance linkage method such that probesets having similar matched pair differences across patients and samples having similar matched pair differences levels were grouped together. A heat map was constructed to depict change in expression level for each patient ranging from blue to red representing expression lower than at new diagnosis to greater than at new diagnosis for relapsed samples respectively for Log₂ transformed robust multi-array analysis (RMA) values; B: probeset level and sample level Mixed Model Analysis of Variance analysis was utilized to compare the expression of the WT1 interactome plus the control genes in B-lineage ALL patients extracted from the in-house RMA normalized database. Fold change and linear contrast P-values are reported for the WT1, CD19 and CD7 probesets