fig5

Figure 5. Augmented expression of WT1 in MLL-R⁺ infant B-lineage acute lymphoblastic leukemia (ALL). A: Expression of log₂ transformed, robust multi-array analysis (RMA) normalized values for 97 MLL-R⁺ infants with B-lineage ALL (GSE68720) were mean centered to the mean expression of 74 normal control samples and clustered using a two-way algorithm (average distance metric) to determine co-regulation of WT1 and the control genes; B: a 3 factor Mixed Model Analysis of Variance analysis was utilized to compare the expression of the WT1 plus the control genes in infant ALL patients with normal samples extracted from the in-house RMA normalized database of 1,556 patients of which nonleukemic control samples and leukemic samples from MLL-R⁺ infant ALL cases were interrogated (5 probesets analyzed to determine least square error of the expression measurements used to calculate P-values). Fold change and linear contrast P-values are reported for the WT1, CD19 and CD7 probesets