fig4

Figure 4. Augmented expression of WT1 in MLL-R⁺ pediatric B-lineage acute lymphoblastic leukemia (ALL). Expression of log₂-transformed, robust multi-array analysis (RMA) normalized values for leukemia samples from 95 MLL-R⁺ patients were mean centered to the mean expression of 74 normal control samples and clustered using a two-way algorithm (average distance metric) to determine co-regulation of WT1 and the control genes. A: The heat map ranging from blue to red represents expression lower than normal to greater than normal in ALL samples respectively for the transformed expression values. CD19 and WT1 were upregulated and CD7 was downregulated in these B-lineage ALL samples; B: expression levels were also examined for the members of the WT1 interactome identified from the HPRD database. A 3 factor Mixed Model Analysis of Variance analysis was utilized to compare the expression of the WT1 interactome plus the control genes in leukemia samples from ALL patients vs. normal samples extracted from the in-house RMA normalized database (36 probesets analyzed to determine least square error of the expression measurements used to calculate P-values). Fold change and linear contrast P-values are reported for the WT1, CD19 and CD7 probesets