fig1

Figure 1. Augmented expression of WT1 in Pediatric T-lineage acute lymphoblastic leukemia (ALL). Expression of log₂ transformed, robust multi-array analysis (RMA) normalized values for primary leukemia cells from 189 newly diagnosed pediatric T-lineage ALL patients were mean centered to the mean expression of 74 normal samples and clustered using a two-way algorithm (average distance metric) to determine co-regulation of WT1 and the control genes. A: The heat map ranging from blue to red represents expression lower than normal to greater than normal in T-lineage ALL samples respectively for the transformed expression values. CD7 and WT1 were upregulated and CD19 was downregulated in T cell ALL samples; B: expression levels were also examined for the WT1 interactome identified from the HPRD database. A 3 factor Mixed Model Analysis of Variance analysis was utilized to compare the expression of the WT1 interactome plus the control genes in T lineage ALL patients with normal samples extracted from the in-house RMA normalized database (36 probesets analyzed to determine least square error of the expression measurements used to calculate P-values) and the most significantly affected expression levels are shown. Fold change and linear contrast P-values are depicted for the WT1, WT1 interactome, CD19 and CD7 probesets