fig4
From: Improved potency of F10 relative to 5-fluorouracil in colorectal cancer cells with p53 mutations
Figure 4. Caspase-8 activity rescue data. HCT116 p53+/+ and HCT116 p53-/- cells were co-treated with indicated concentrations of (left) F10 or (right) 5-FU along with indicated nucleoside (uridine = 1 mmol/L, thymidine = 80 μmol/L) for 48 h. Caspase activity per cell viability was determined using Promega's Caspase-Glo assay after 48 h and calculated following blank subtraction relative to respective control activity. Experiments done in triplicate ± SEM. P-values calculated using GraphPad Prism (n = 4; *P ≤ 0.05, ***P ≤ 0.001)
