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Figure 3. circNCOA3 promotes CRC progression. (A) Levels of circNCOA3 were measured by RT-qPCR in normal colon cells (NCM460) and various CRC cell lines; (B) Levels of circNCOA3 in CSC cell lines HCT116 and SW620 transfected with shRNAs; (C and D) Proliferation assays in cell lines HCT116 (C) and SW620 (D) transfected with sh-circNCOA3; (E) Colony formation assays of the CRC cell lines HCT116 and SW620 transfected with sh-circNCOA3; (F) Transwell assay of the CRC cell lines HCT116 and SW620 transfected with sh-circNCOA3; (G) Tumor growth in C57BL/6 mice implanted with MC38-sh-NC or MC38-sh-circNCOA3 cells; (H) Tumor growth in BALB/c nude mice implanted with MC38-sh-NC or MC38-sh-circNCOA3 cells; (I) The OS in different groups analyzed using the Kaplan-Meier method. CRC: Colorectal cancer; RT-qPCR: real-time polymerase chain reaction; OS: overall survival.