Cellular irinotecan resistance in colorectal cancer and overcoming irinotecan refractoriness through various combination trials including DNA methyltransferase inhibitors: a review

Changes in the expression levels of drug efflux transporters in human colon cancer cells

Overexpression of ATP-binding cassette drug efflux transporters (ABC transporters, such as ABCB1 or MDR1) on the plasma membrane of cancer cell lines, isolated as paclitaxel- or olaparib-resistant cells, has been regarded as a mechanism of drug resistance toward relatively lipophilic anti-cancer drugs, such as doxorubicin, rucaparib, paclitaxel, and olaparib, for a long time^[9]. Overexpression of a second ABC transporter, ABCG2 [breast cancer resistance protein (BCRP)], is more significant in irinotecan resistance^[10-13]. Different research groups have independently established ABCB1-^[12,13] and/or ABCG2-overexpressing^[10-12] cancer cell lines. As illustrated in Figure 2, higher resistance factors were observed for SN-38 (approximately 50-fold), irinotecan (17-48-fold), and topotecan (approximately 40-fold) in ABCG2-overexpressing cells compared to ABCB1-overexpressing cells. On the other hand, an increased level of resistance was observed for anthracyclines (38-52-fold) in ABCB1-overexpressing cells compared to ABCG2-overexpressing cells. ABCG2 cDNA-mediated expression in human osteosarcoma Saos-2 cells made them highly resistant (approximately 100-fold) to irinotecan and SN-38^[14]. Maliepaard et al.^[15] established a topotecan-selected and topotecan-resistant human ovarian cancer cell line, T8, which showed 176-fold resistance to SN-38 and 11-fold resistance to mitoxantrone from the human IGROV1 ovarian cancer cell line as the parental cell. They also demonstrated that the resistance was due to increased topotecan efflux, which led to decreased intracellular accumulation of the drug^[15]. The drug-resistant T8 subline was shown to overexpress the BCRP/mitoxantrone resistance/placenta-specific ABC transporter (BCRP/MXR/ABCP) gene^[15]. Owatari et al.^[16] reported that copper-transporting P-type ATPase, ATP7A, confers drug resistance to SN-38, irinotecan, paclitaxel, vincristine, doxorubicin, etoposide, and mitoxantrone by ATP7A cDNA-mediated expression in ovarian cells of a Chinese hamster and immortalized fibroblasts from a patient with Menkes disease.

Figure 2. Factors causing resistance to anti-cancer drugs in ABCB1- or ABCG2-overexpressing colon cancer cell lines and an ABCB1-overexpressing HeLa cell line. Resistance factors for SN-38, irinotecan, topotecan, mitoxantrone, anthracyclines, 5-fluorouracil, and platinum anticancer drugs are shown relative to the respective parental cell lines. Cell lines are HCT116-SN6 [2004 (COL), ABCG2-overexpressing colon cancer cell^[10] (orange)], S1-IR20 [2021 (COL), ABCG2-overexpressing colon cancer cell^[11] (gray)], S1-B1-20 [2000 (COL), ABCB1-overexpressing colon cancer cell^[12] (yellow)], and Hvr100-6 [2002 (HeLa), ABCB1-overexpressing HeLa cell^[13] (blue)]. Establishments of these resistant sublines are described in Table 1. ND: Not determined.

Until recently, many studies have shown that ABC transporter expression is regulated by epigenetic modifications or microRNA (miRNA) regulation. Hypermethylation of the ABCG2 promoter decreased its mRNA levels. Demethylation by a DNA methyltransferase (DNMT) inhibitor was reported to recover mRNA expression and induce drug resistance^[19]. H3K4me3 demethylation increases the expression of ABCC1 mRNA and protein^[20]. Multidrug-resistant (MDR) sublines from MCF-7, IGROV1, and S1 cell lines were established from treatment with low doses of either etoposide or doxorubicin; thus, ABCG2 expression was increased in the resistant cells. Considerably higher enrichment (approximately 10-fold) of acetylated histone H3 and RNA polymerase II to the proximal ABCG2 promoter has been detected in the low-dose doxorubicin-selected MCF-7 cells (1.3-3.6-fold resistant to doxorubicin) than in the parental MCF-7 cells. In contrast, a lower association of histone deacetylase 1-ABCG2 promoter was detected in cells with low-dose doxorubicin-selected MCF-7 cells^[17]. The expression levels of some miRNAs have also been reported to correlate with ABCG2 expression; miR-181a^[21], miR-328^[22], and miR-487a^[23] (breast) and miR-519c^[18] and miR-520h^[24] (colon) were base-paired to ABCG2 mRNA 3’-UTR and the binding silenced ABCG2 expression. Drug-resistant breast cancer cell lines showed low expression levels of these miRNAs. miR-519c, whose target site in the 3’-UTR existed only in the parental S1 cells, was thought to be involved in indirect regulation through an mRNA-binding protein, HuR, resulting in the loss of stability of the ABCG2 mRNA molecules in the parental cells^[24]. ABCG2 overexpression in a drug-resistant subline, S1-M1-80, which is derived from the S-1 cell line, lacked a binding target site for miR-519c in its 3’-UTR sequence of ABCG2 mRNA (i.e., short ABCG2 3’-UTR) and is likely to result in high ABCG2 expression^[24]. Clinically, tumors resected from 17 out of 26 CRC patients, who were responsive to 5-FU-based chemotherapy, showed remarkably lower ABCG2 levels than in adjacent normal tissues. In contrast, some unresponsive patients treated with 5-FU-based chemotherapy had comparable or even higher tumoral expression of ABCG2 than in the adjacent normal tissues. Most of the unresponsive patients exhibited a high expression of tumoral ABCG2 and HuR simultaneously^[18]. Further clinical analyses revealed that tumors from three out of nine unresponsive patients carried a short ABCG2 3’-UTR in the mRNA, which prevented miR-519c-mediated repression and led to overexpression of ABCG2 mRNA^[18]. Figure 3 shows these cellular events in a schematic diagram.

Figure 3. Epigenetic regulation mechanisms in cancer cells that result in acquired irinotecan resistance. Methylation in a promoter region of a drug efflux transporter, ABCG2, and stabilization of a mRNA binding protein, HuR, through miR-519c function are depicted as epigenetic regulation mechanisms. When positive regulation mechanisms are prevailing in cancer cells, ABCG2 gene expression will be enhanced. Thus, cancer cells will become resistant to irinotecan.

Elevated cellular drug metabolism in relation to pregnane X receptor or steroid and xenobiotic receptor

It has been reported that irinotecan undergoes hepatic metabolism to be converted to CYP3A4-dependent oxidative metabolites, and it is then converted by carboxylesterases to an active metabolite SN-38. Next, the active SN-38 undergoes glucuronide conjugation to be an inactive metabolite, SN-38G. The induction of SN-38 glucuronidation within CRC cells renders the cancer cells resistant to irinotecan-based therapy. Pregnane X receptor (PXR) and steroid and xenobiotic receptor (SXR) are well-known since they are expressed mainly in mammalian liver and gastrointestinal tract. Moreover, they are involved in the induction of various classes of drug-metabolizing enzymes and drug transporters to detoxify xenobiotics and therapeutic drugs. cDNA-mediated expression of human PXR in cultured human colon cancer cells, LS174T, made the cells resistant to SN-38 by enhancing the glucuronidation of SN-38 to SN-38G. Conversely, suppression of PXR expression by short-hairpin RNA-expressing vectors decreased the intracellular and extracellular SN-38G/SN-38 ratios, in accordance with UGT1A1 downregulation^[25]. Furthermore, SN-38 was shown to activate PXR in human colon cancer cell lines LS180 and HCT116 and induce CYP3A4, CYP3A5, UGT1A1, and the ABC transporter MRP2^[26]. The levels of PXR and SXR expression are the highest in human livers, followed by intestinal tissue. Therefore, activation of PXR/SXR would result in the induction of various drug-metabolizing enzymes and drug transporters, all of which play a role in the disposition and excretion of irinotecan, SN-38, and SN-38G [Figure 4]. In fact, herb-irinotecan interactions have also been reviewed, where St. John’s wort is administered with anti-cancer drugs because it was suggested to exert mild to moderate effects against depression^[27]. These results, together with the background studies on PXR/SXR, are summarized in Table 2.

Figure 4. Elevated cellular drug metabolism in relation to pregnane X receptor or steroid and xenobiotic receptor. Induction of glucuronidation of SN-38 within colorectal cancer cells renders cells resistant to irinotecan-based therapy. PXR and SXR are well known as they are expressed mainly in mammalian livers and gastrointestinal tracts and are involved in the induction of various classes of drug-metabolizing enzymes and drug transporters to detoxify therapeutic drugs such as irinotecan and SN-38. PXR: Pregnane X receptor; SXR: steroid and xenobiotic receptor; RXR: retinoid X receptor.

Changes in drug targets

The molecular targets of the active metabolite of irinotecan, SN-38, are Top1-DNA covalent reaction intermediates that are reversibly stabilized by SN-38 and result in DNA strand breaks [Figure 5]^[28]. DNA damage by irinotecan has been documented in regards to an increase in DNA damage by the inhibition of proteins, such as Rad51 and Rad52, which are involved in DNA repair. Curcumin was reported to target homologous recombination through the inhibition of Rad52, resulting in the sensitization of irinotecan-inducing DNA damage^[29]. Flavopiridol, a cyclin-dependent kinase inhibitor, was shown to be beneficial to patients with advanced solid tumors that expressed wild-type p53 treated with irinotecan through the suppression of Rad51^[30]. Resistance in Top1-targeting drugs has been attributed to qualitative and quantitative alterations of Top1. Since the properties of drug resistance vary widely, we summarize these research results in Table 3^[31-37]. Generally, irinotecan-resistant cells showed lower Top1 activity or protein levels. Variable copy numbers of Top1 gene were reported in five different cultured human cancer cells [Table 3]. The Top1 gene locus in chromosome 20q has been shown to be apt to undergo chromosome alterations that result in gene dosage changes, as the locus is in proximity to an oncogene documented as “chromosome 20 deletions in myeloid malignancies”^[38].

Figure 5. Factors affecting levels of the Top1-DNA cleavage complex and irinotecan resistance. Levels of irinotecan-causing DNA damage response depends upon formation of the Top1-DNA cleavage complex through Top1 function. Decreases in cellular Top1 activities, Top1 gene copy number, and Top1 protein levels result in cellular irinotecan resistance. Top1: Topoisomerase I.

Copy number variations of the Top1 gene were extensively investigated in normal mucosa and tumor tissue samples from 50 CRC patients. The analyses were performed using a fluorescence in situ hybridization probe mixture covering the Top1 gene and a chromosome 20 centromere (CEN-20). The results show that 32 out of 50 CRC samples had a Top1/CEN-20 ratio higher than those observed in the unaffected colorectal mucosa, and that 42 out of 50 samples had increased Top1 gene copy number^[39]. Top1 has been reported as a predictive biomarker: irinotecan and oxaliplatin failed to treat patients with low Top1 but yielded major improvement in patients with moderate/high Top1^[40]. Furthermore, the same clinical study indicated that patients with a high Top1 showed major improvement in overall survival (OS) with first-line combination chemotherapy (5-fluorouracil combined with irinotecan or oxaliplatin)^[40]. It was reported that the germline mutation frequency in Top1 gene was so low that it was not detected in 236 subjects or in 16 untreated lung cancer tissues. These results suggest that Top1 gene mutation seldomly occurs in human subjects and human cancer tissues^[41].

Transition of colon cancer cell phenotypes for acquiring irinotecan resistance

While irinotecan-resistant cancer cells are generally established by a stepwise dose escalation in an in vitro cell culture system, cancer cells in vivo are highly heterogeneous in CRC tumor mass. In this section, alterations in CRC cell phenotypes are discussed during cancer progression. A variety of biological factors may affect cancer cell phenotypes including cancer cell stemness, cell proliferation, metastasis, cell dormancy, and drug resistance. The G-protein coupled chemokine receptor, CXCR4, is highly expressed in CRC and has been shown to be associated with cancer stemness, metastasis, and poor prognosis. Furthermore, it has been shown that the CXCR4/CXCR7 pathway is activated when CXCL12 binds to either receptor^[42]. In particular, CXCR4 receptor-ligand binding is thought to be associated with cancer stemness, development of metastasis, and poor prognosis. CXCR4 has been shown to co-localize with CRC stem cell markers, such as Lgr5, CD133, and CD44, which are thought to be associated with the epithelial-mesenchymal transition process and resistance to cancer drug therapy [Figure 6]^[42].

Figure 6. Transition of colon cancer cell phenotypes in relation to alteration of status of CXCR4 and CRC markers for acquiring irinotecan resistance. The G-protein coupled chemokine receptor, CXCR4, co-localizes with CRC stem cell markers, such as Lgr5, CD133, and CD44, which are thought to be associated with the epithelial-mesenchymal transition process and resistance to cancer drug therapy. CSC: Cancer stem cell; SDF-1α: stromal cell-derived factor-1α; TGFα: tumor growth factor-α; ERK1/2: extracellular signal-regulated kinase 1/2.

The expression of CXCR4 in colon cancer cells after treating with stromal cell-derived factor-1α (SDF-1α, or CXCL12) was investigated, along with the cancer cell proliferation rate and chemosensitivity to 5-FU, irinotecan, and oxaliplatin. This was carried out using high and low CXCR4-expressing human colon cancer HT29 sublines and SW480 cells with or without lentiviral CXCR4 overexpression^[43]. Treatment with SDF-1α activated extracellular signal-regulated kinase 1/2 and stimulated cell proliferation. Higher expression levels of CXCR4 were associated with increased drug sensitivity to 5-FU, irinotecan, and oxaliplatin, compared to their low-CXCR4-expressing counterparts. Conversely, colon cancer cells with low CXCR4 levels were resistant to the tested anti-cancer agents, indicating that cancer cell dormancy is associated with drug resistance^[43]. Another study showed that chemotherapeutic drugs, including SN-38, vinblastine, and methotrexate, decreased the population of CXCR4⁺/CD26^- cells and increased the CXCR4^-/CD26⁺ cell population^[44]. These two independent studies were consistent in that CXCR4⁺ cells were more sensitive to chemotherapeutic effects, and thus were preferentially eradicated; as a result, the CXCR4^- cell population became more prevalent. Gongora et al.^[45] also reported that a drug-resistant subline of human colon cancer, i.e., HCT116 cells (HCT116-SN6), which were 6-8-fold more resistant to SN-38, proliferated more slowly than the parental cell line (HCT116-s)^[10,45]. Thus, tumor dormancy associated with CXCL12-CXCR4 signaling may be related to a drug-resistant phenotype in human colon cancer cells, which requires further investigation.

In addition, A/J mice who were fed a Western diet (WD, 20% fat; Normal, 5% fat) revealed WD-inducible metalloprotease (ADAM17) activation through CXCL12-CXCR4 signaling. ADAM17 activation, which is linked to the activation of epidermal growth factor receptors, increases CXCL12 in stromal cells and tumor growth factor-α (TGF-α) in colonocytes. This increase in the levels of CXCL12 and TGF-α is associated with an increased risk of colon cancer development^[46].

The tumor microenvironment may affect cancer cell properties and drug sensitivity

There are many important findings on acquired cellular irinotecan resistance with enhanced efflux transporter activities, as well as the qualitative and quantitative changes in Top1. Irinotecan sensitivity or effectiveness might differ between a certain human colon cancer cell line and cancer xenografts of the same cell line, which might also be different between clinical CRCs consisting of cells with similar properties, even if pharmacokinetically equivalent irinotecan exposure was established between these experimental and clinical models. We reported that human liver cancer cell lines undergo differential gene regulatory mechanisms in two-dimensional (2D) plastic plate cultures and three-dimensional (3D) spheroid cultures^[47]. In 2D culture, a nuclear receptor, aryl hydrocarbon receptor (AhR), mainly regulates CYP1A1 and CYP1A2. In 3D cultures, AhR mainly regulates CYP1A1, but not CYP1A2. Instead, PXR is involved in the regulation of CYP1A1, CYP1A2, or both, depending on the human liver cancer cell line^[47]. Thus, the tumor microenvironment is very likely to affect gene regulatory mechanisms and other cellular functions. Our 3D culture systems seem to be extremely useful as a research tool for studying the tumor microenvironment in CRC cell lines. One important point to be considered is the difference in extracellular environments around individual cancer cells. D’Angelo et al.^[48] created new tissue culture models to study colorectal cancer liver metastasis (CRLM) by recruiting 18 CRLM and 5 CRC patients. The investigators conducted decellularization of healthy colon (HC), CRC, healthy liver (HL), and CRLM samples. They conducted Masson’s trichrome staining before and after decellularization. Human CRC HT-29 cells were cultured in patient-derived decellularized scaffolds derived from CRC and CRLM samples. Both scaffolds supported cell viability. In colorectal tissues, HT-29 cell viability was better in CRC than in HC samples. Investigation of the epithelial-mesenchymal transition profile revealed a significant reduction in E-cadherin expression and a significant increase in vimentin expression when HT-29 cells were cultured in CRLM scaffolds compared to HC, HL, and CRC. In the case of HCT116 cell culture in CRC and CRLM samples, E-cadherin and vimentin were expressed without any remarkable difference in HC, HL, CRC, or CRLM. Importantly, the 3D environment created with the patient-derived scaffolds showed a tendency to reduce the cellular sensitivity to 5-FU and FOLFIRI in comparison to those in 2D environments. Nevertheless, 3D-cultured HT-29 cells in HL scaffolds resulted in a significant reduction in cell proliferation following FOLFIRI treatment. The cytotoxic effects of FOLFIRI were not remarkable in HT-29 cells in a 3D CRLM scaffold culture. Cellular drug sensitivities influenced by the tumor microenvironment seem to vary between different cancer cells and anti-cancer drugs [Figure 7].

Figure 7. The tumor microenvironment may affect cancer cell properties and drug sensitivity/resistance. Scaffolds of cancer tissues were prepared by decellularization. Human colorectal cancer cells, HT-29, were inoculated on the resultant scaffolds to form 2D or 3D cultures of HT-29 cells. HT-29 cells in the 3D culture systems were created using scaffolds from healthy liver or colorectal cancer liver metastasis tissue, which showed overall resistance compared to 2D-cultured HT-29 cells. 3D-HT-29 cells on CRLM scaffolds were resistant to 5-fluorouracil. PBMC: Peripheral blood mononuclear cell; CRLM: colorectal cancer liver metastasis; HL: healthy liver; 5-FU: 5-fluorouracil; TGF-β: transforming growth factor-β; IL-10: interleukin-10; Treg: regulatory T cell.

Another example of the tumor microenvironment association with drug resistance is extracellular vehicles (EVs), which mediate intercellular communication between cancer cells. EVs can contain small molecules such as therapeutic drugs, mRNAs, miRNAs, or even proteins, such as drug efflux transporters^[49,50]. Exosome-mediated transfer of circular RNA (circ_0000338) enhanced 5-FU resistance in circular RNA-transmitted human CRC cell lines, which was achieved through its direct binding with miR-217 and miR-485-3p. Serum concentrations of circ_0000338 were higher in CRC patients who were resistant to the 5-FU regimen, which is consistent with the results of in vitro exosome-mediated circ_0000338 transfer [Figure 7]^[51].

Recent investigations have also shown that the expression of some miRNAs in colon cancer HT-29 cells may alter the colon cancer microenvironment and cell-to-cell interaction. miR-146a was overexpressed in HT-29 cells and co-cultured with peripheral blood mononuclear cells (PBMCs). miR-146a overexpression increased transforming growth factor-β and interleukin-10 expression in PBMCs. In a co-culture system of miR-146a, overexpressing HT-29 cells with PBMC enhanced the population of regulatory T cells (Tregs) and induced irinotecan and 5-FU resistance in HT-29 cells^[52]. The underlying mechanisms of an increased population of Tregs and drug resistance in HT-29 cells remain to be determined.

The effects of the tumor microenvironment on cancer cell properties are summarized in Table 4.

Colon cancer stem cells/colon cancer-initiating cells and drug-resistant phenotypes

Stem cells are defined as cells that can renew themselves, i.e., self-renew, and generate mature cells by differentiation to form their respective tissues. A well-known example is hematopoietic stem cells, which divide extensively in the bone marrow and are differentiated into common myeloid progenitors, erythrocytes, leukocytes, platelets, macrophages, and granulocytes, as well as into common lymphoid progenitors, B cells, T cells, natural killer cells, and dendritic cells. Stem cells often become targets of genetic events, resulting in malignant transformation. In abnormal processes, where signal transduction fails to function properly, stem cells may be dysregulated, leading to uncontrolled self-renewal and malignant transformation^[53]. In colon cancer, Ricci-Vitiani et al.^[54] reported that tumorigenic cells in colon cancer existed as a high-density CD133⁺ population. The CD133⁺ cell population was present in approximately 2.5% of the total tumor cells. Tumorigenic CD133⁺ cells subcutaneously implanted in immunodeficient mice reproduced the original tumor properties. In contrast, CD133^- cells did not develop tumors^[54]. Fang et al.^[55] reported that freshly isolated CD133⁺ colorectal adenocarcinoma cells from patients undergoing surgical resection were maintained for longer periods in tumor spheres, or so-called spheroids, and achieved long-term maintenance of CD133⁺ phenotype, retaining CSC properties. These isolated cells formed spheres under the adjusted medium for CSC culture. On the other hand, the cells could be induced to a differentiated state to lose CD133 expression in Dulbecco’s modified Eagle’s medium supplemented with 20% fetal bovine serum in collagen I-coated flasks. Importantly, treatment with irinotecan resulted in only 25% inhibition of clonogenicity of colon tumor spheroid cells, whereas 75% inhibition of clonogenicity of differentiated (adherent) population occurred. While more than 50% of the differentiated cells underwent 2 mM irinotecan-induced growth inhibition, 40% of the spheroid cells were resistant to irinotecan even at a concentration of 135 mM. These results clearly indicate that undifferentiated spheroid cells were more resistant to irinotecan than differentiated and adherent cells^[55]. Altogether, in human colon cancer tissues, colon CSCs express CD133, a rare population of undifferentiated cells capable of self-renewal and maintaining the mass of colon cancer and drug-resistant phenotype.

Several investigators have reported drug-resistant phenotypes associated with increased activities or expressions of drug-metabolizing enzymes, drug transporters, and transcriptional regulators of gene products that function in drug metabolism and disposition. An in vitro colon CSC model was created by serum-free cell culture of human COLO 205 cells in a stem cell medium containing basic fibroblast growth factor, epidermal growth factor, and insulin-transferrin-selenite solution. Expression of CD133 was ascertained in CSC-like cells, and the CSC-like cells achieved approximately 15-, 10-, and 3.5-fold higher CD133, CYP3A4, and aldehyde dehydrogenase 1A1 (CSC marker) expression, respectively, compared to the control COLO 205 cells cultivated in standard cell medium (supplemented with 10% fetal bovine serum)^[56]. The irinotecan-resistant phenotype emerged in the human colon cancer cell line LoVo, where a leucin-rich repeat-containing G-protein coupled receptor 5 (Lgr5) underwent siRNA-mediated knockdown. Lgr5 knockdown resulted in the expression of GPR56, a member of the adhesion G-protein coupled receptor subfamily. Reportedly, GPR56 is highly expressed in colon cancer cells. GPR56 has been shown to be expressed in normal intestinal crypt stem cells and has been identified as a marker of a subgroup of acute myeloid leukemia CSCs. Lgr5⁺ CRC cells and Lgr5^- cancer cells are known to be interconvertible. As already mentioned, Lgr5^- cells are regarded as resistant to irinotecan and 5-FU^[57]. siRNA-mediated Lgr5 downregulation and GPR56 overexpression lead to increased expression of MDR1 (P-glycoprotein), which appears to be associated with irinotecan resistance^[57]. Another study employed patient-derived CRC cells with CSC characteristics of self-renewal (i.e., forming spheroids) and resistance to chemotherapeutic agents. Patient-derived spheroids contained a higher proportion of cells with high aldehyde dehydrogenase (ALDH) activity, indicating CSC-like properties. Spheroidal cells showed a greater resistance (approximately 2-fold with statistical significance of P < 0.01, based on EC₅₀) to a combination of 5-FU and SN-38, as compared to the same cells kept as adherent monolayers. Higher expression of PXR and colon CSC markers, including ALDH1A1, Oct-4, and Lgr5, along with PXR target genes (CYP3A4 and ABCG2), was observed in spheroids as compared with cells in 2D conditions [Figure 8]. Colon cancer cells resected from stage II and III patients showed a statistically significant positive correlation between PXR and ALDH1A1 mRNA levels. Moreover, stage II patients treated with 5-FU-based chemotherapy, who had tumors with higher PXR expression, showed poor prognosis^[58]. These examples of CSC-associated drug resistance are summarized in Table 5.

Figure 8. Cancer stem cells within a colorectal cancer tissue and drug resistance or drug sensitive phenotype. Tumorigenic cells in colon cancer existed as a high-density CD133⁺ population. Small population of CD133⁺ cells that are tumorigenic and drug resistant when subcutaneously implanted in immunodeficient mice reproduce the original tumor properties. In contrast, CD133^- cells do not develop tumors and show drug sensitive phenotype. CSC: Cancer stem cell.