1Department of Life Sciences,
2Department of Medicine, Surgery and Neuroscience,
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Cancer is the second leading cause of death worldwide. The survival of cancer patients depends on the efficacy of therapies and the development of resistance. There are many mechanisms involved in the acquisition of drug resistance by cancer cells, including the acquisition of stem-like features. Cancer stem cells (CSCs) represent a major source of tumor progression and treatment resistance. CSCs are a subpopulation of cancer cells having the abilities to self-renew and form spheres in vitro. Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cytosolic enzyme involved in the detoxification of cells from toxic aldehydes and belongs to the ALDH family. High ALDH1A1 activity is closely related to stemness phenotype of several tumors, possibly contributing to cancer progression and diffusion in the body. We have documented the contribution of ALDH1A1 in tumor angiogenesis in breast cancer cells by the activation of hypoxia inducible factor-1α and vascular endothelial growth factor signaling. This review discusses the involvement of ALDH1A1 in the development of different hallmarks of cancer to propose it as a novel putative target for cancer treatment to achieve better outcome. Here, we analyze the involvement of ALDH1A1 in the acquisition of stemness phenotype in tumor cells, the regulation of tumor angiogenesis and metastases, and the acquisition of anticancer drug resistance and immune evasion.
Aldehyde dehydrogenase 1A1, angiogenesis, drug resistance, immune editing, metastases, stemness
Cancer is highly dynamic as tumor cells become more heterogeneous during the progression of the disease. Twenty years ago, Hanahan and Weinberg published a milestone review, in which they organized the complexity of tumor biology into six major hallmarks, including cell stemness. An updated review from 2011 by the same authors added two emerging hallmarks: reprogramming of energy metabolism and evading immune destruction. The translation of hallmarks into clinical practice has been proved by the development of drugs associated with specific hallmarks, but the view of their complementarity is necessary to explain the heterogeneity of cancer.
Tumor cells in the neoplastic mass, characterized by enhanced capacity of self-renewal and elevated ability to seed new tumors upon injection into new hosts, are termed cancer stem cells (CSCs). For these features, CSCs have also been referred to as “tumor-initiating cells” or “sphere-forming cells”. CSCs can be implicated in many hallmarks of cancer and recently CSC-directed therapies have been clinically developed. The involvement of these cells in chemoresistance is known, in particular by the maintenance in G0 phase; high expression of drug efflux pump, anti-apoptotic proteins, and scavenger molecules; more efficient DNA repair; and a protective microenvironment created by interaction with the tumor niche[5,6].
The gold standard model to define CSCs is the serial dilution in vivo transplantation, but several markers of cell surface and intracellular compartment have been identified. Aldehyde dehydrogenase 1A1 (ALDH1A1) is a protein that has been used to mark CSCs in several cancers, including leukemias and carcinomas of the breast, colon, liver, lung and pancreas, among others. This enzyme belongs to the ALDH superfamily, composed of nicotinamide adenine dinucleotide (phosphate) (NAD(P)+)-dependent enzymes that catalyze the oxidation of exogenous and endogenous aldehydes to the respective carboxylic acids.
Overproduction of reactive oxygen species (ROS) in cells may cause oxidation of polyunsaturated fatty acids in the cellular membrane through free radical chain reactions with the formation of aldehydes as final products, which play a crucial role in the pathogenesis of various chronic and acute diseases such as cancer, inflammation, and atherosclerosis, among others[8,9]. However, aldehydes are ubiquitously distributed in the environment and hence they can be acquired from different sources such as food, water, and air. By preventing the accumulation of aldehydes derived from endogenous or exogenous processes, ALDHs mitigate oxidative damage at the cellular and tissue levels. In humans, there are 19 isoforms of ALDH with different cell compartmentalizations and specific tissue distributions. The ALDH1 family, especially ALDH1A1 and ALDH1A3 isoforms, is required for retinoic acid (RA) biosynthesis. The role of ALDH1A1 as robust marker of stemness has been reported in depth by several authors, but in the last decade the emerging role in tumor progression of this enzyme has been proposed. Tumor cells are characterized by an aberrant redox state and an increase of ALDH1A1 expression can be helpful as ROS scavenger. Further, the metabolic activity of ALDH1A1 is strictly related to inactivation of anti-cancer drugs, including oxazaphosphorines or aldophosphamide, in nontoxic metabolite and the expression of this enzyme is a marker of poor prognosis in many tumors. The silencing of the ALDH1A1 gene in human breast cancer cells increases their sensitivity to paclitaxel with a concomitant increase of ROS formation, and similar results were obtained with doxorubicin, sorafenib, and staurosporine. The opposite effect is observed when ALDH1A1 is present. It has been proposed that cancer cells acquire drug resistance by inducing ALDH1A1 expression. Indeed, increased ALDH1A1 expression in relapsing tumors of human breast cancer after surgery and tamoxifen treatment has been observed. ALDH1A1 expression in breast cancer has also been associated with advanced disease stages, triple negative cells, and poor prognosis. Although several biological functions are mediated by RA, the molecular mechanism by which ALDH1A1 regulates cellular functions in oncology is not clear.
In the present review, we describe the contribution of ALDH1A1 in tumor stemness and cancer progression, analyzing its involvement in angiogenesis, tumor spread, drug-resistance, and immune-evasion.
A search was conducted using the terms listed in Table 1 in the PubMed bibliographic database, including original articles, reviews, and clinical data belonging to the experimental area, written in English, and published from January 2000 to September 2019 in journals with high impact factors. Useful papers published before January 2000 were also included. We performed a narrative synthesis of the results reported in the selected manuscripts.
Main and secondary keywords used for literary search
|Main key words||Secondary key words|
| Preclinical study
Cancer is the result of oncogenic mutations with survival advantages over other cells. During tumorigenesis, any cell is susceptible to malignant transformation depending on the accumulation of oncogenic mutations. Recently, it has been recognized that the majority of cancers are due to random mutations arising during DNA replication in stem cells (SCs) and only a third are attributable to environmental factors or inherited predispositions.
In the last years, the concept of CSCs has received increasing interest in tumor progression and regeneration. It is thought that cancer recurrence could be mediated by these CSCs. They are a subpopulation of cancer cells having the ability of self-renewal as well as tumor-initiating capacity. In addition, they are now recognized to play a crucial role in tumor metastases, relapse, and chemo/radio-resistance[19,20]. Indeed, cancer cells with stem phenotype acquire a quiescent state, cell cycle arrest, and lower response to chemotherapeutic agents[21,22], as well as to novel therapies such as immunotherapy and anti-angiogenics. As tumor initiators, CSCs are considered to be a promising target for obtaining a better therapeutic outcome. Nowadays, cancer treatment has been reoriented toward the understanding of CSCs’ intrinsic properties and mechanisms that they develop to survive and enhance their aggressive phenotype.
The discovery of CSC markers has facilitated their isolation and characterization. They have been identified in blood, breast, colon, melanoma, pancreatic, prostate, ovarian, and lung cancers, among others. In recent years, many molecules have been proposed as CSC biomarkers. More than 25 transcriptional factors codify for surface and intracellular stemness markers. CD44 is a transmembrane glycoprotein that plays an important role in cell division, migration, adhesion, and signaling. It is expressed in many tumors, including bladder, breast, colon, gastric, glioma, head and neck, osteosarcoma, ovarian, pancreatic, and prostate cancers, as well as leukemia. CD133 is another transmembrane glycoprotein localized in cellular protrusions that regulates stem-like features of many cancers. In addition, several stemness markers are not localized on the cell surface. ALDH1A1 is a robust marker of these cells in many tumors and it could be used to enrich tumor-initiating subpopulations from various cell lines and primary tumors[25-30].
ALDH1A1 belongs to the ALDH1 family and is a marker of normal SCs and CSCs. In cell cultures, the activity of this class of enzymes is measured by Aldefluor kit that determines the total activity of ALDH1 family. Fluorescent substrate of enzymes are used to quantify ALDH activity. This probe is retained in the cells and the amount of fluorescent reaction product, measured with flow cytometer, is proportional to ALDH activity. ALDH1A1 activity is associated to poor clinical outcome in lung, esophagus, stomach, ovarian, breast and colorectal cancer patients[26,32-36]. ALDH1A1 is a cytosolic enzyme involved in detoxification of acetaldehyde deriving from ethanol metabolism, similar to ALDH2, which is preferentially located in mitochondria. These enzymes are mainly responsible for acetate production in the cells and counteract alcohol-related carcinogenesis. Moreover, the ALDH1 family catalyzes the formation of RA from retinaldehyde. Retinoic acid binds retinoic acid receptor or retinoic X receptor, which transcriptionally regulates many downstream developmental genes in physiological and pathological conditions[38,39]. Indeed, retinoids are involved in the pathogenesis of cancer, obesity, diabetes, and cardiovascular diseases, where the metabolism of these compounds is strictly regulated by the activity of the ALDH1 family, specifically isoforms A1-A3. Luo et al. demonstrated that the RA-target genes and RA responsive elements are characteristic traits of melanoma CSCs from patients. ALDH1A1 can also maintain CSC phenotype by preventing apoptosis through ROS level regulation.
Besides the catalytic activity, ALDH1A1 exerts many biological functions via non-catalytic pathways, for example through the binding to hormones (e.g., androgen, thyroid hormone, and cholesterol), small molecules, and xenobiotics (e.g., daunorubicin and quinolone). However, ALDH1 is mostly involved in stemness phenotype of tumor through its catalytic activity. CSCs exhibit elevated ALDH1A1 catalytic activity, and metabolic inactivation of selected anti-cancer agents by such activity is believed to underlie chemoresistance and consequent cancer recurrence.
In this section, we describe the effects of ALDH1A1 in stemness phenotype tumor angiogenesis, tumor metastases, antitumor drug resistance, and immune evasion, hypothesizing its central role in tumor malignancy.
The transformation of SCs into CSCs, cancer progenitor cells, is an important step in carcinogenesis as, after CSC development, they remodel the tissue niche and produce a pathological cancer microenvironment termed “CSC niche”. The CSC niche is responsible of four main functions, namely anchorage, survival, protection, and proliferation, in addition to the maintenance of cancer stemness and plasticity and the editing of the immune system. Cells composing the CSC niche are important for CSC maintenance and the generation of factors further involved in invasion, metastases, and promotion of angiogenesis[44-46]. ALDH1A1 is historically considered a pivotal regulator of CSCs. In 2014, Menendez and Alarcon coined the term “metabostemness” to indicate the orchestration of the genetic reprogramming that redirects normal and tumor cells toward less-differentiated CSC states. The stemness state is not permanent because there is a dynamic and bidirectional interconversion between CSCs and non-CSCs, depending on their relative abundance; in other words, not only can cancer cells become CSCs, but the reverse process can also happen. Accordingly, ALDH1A1 is a CSC marker of many tumors, but it is also expressed in tumor cells in a more differentiated state, characterized by lower or limited proliferative potential[48,49]. This plasticity may depend on the niche where CSCs live. The CSC niche is represented with a specific microenvironment that could decide the fate of CSCs and be an important way to overcome cellular resistance and prevent metastases. Despite accumulating evidence on the functional role of ALDH1A1 in stemness, the specific mechanisms involved in its regulation in CSCs remain unclear. In recent years, different in vitro assays have been developed to evaluate stemness phenotype and metastatic potential of tumor cells[52-54]. In particular, transwell assay and tumorsphere growth in ultra-low attachment plates are correlated to tumorigenicity and stemness. Identification of CSCs by measuring ALDH1A1 activity has been analyzed by Aldefluor assay®. However, this test also identifies ALDH1A2 and ALDH1A3 isoforms[31,39], suggesting a contribution of these other isoforms in the stem-like features of tumor cells. Since the ALDH superfamily comprises many isozymes characterized by specific tissue distribution, it is possible to speculate that different ALDH isozymes contribute to the maintenance of CSCs in different cancers. This speculation is supported by several pieces of evidence, demonstrating a specific elevated expression of ALDH1B1 in colon cancer; ALDH3B1 in lung, breast, ovarian, and colon cancer; ALDH3A1 in lung cancer; and ALDH7A1 in prostate cancer. Such information is critical for understanding the biological significance of the ALDH isozymes in the tumor cells and, as a consequence, for the development of anti-cancer therapies targeting ALDHs.
ALDH1A1 has recently been positively correlated with several in vitro tumor cell behaviors that are surrogates of cancer progression. Yang et al. demonstrated that acquisition of invasive and metastatic capabilities correlated with epithelial-mesenchymal transition phenotype in ALDH1A1high esophageal squamous cell carcinoma. Consistently, ALDH1A1high glioma cells showed higher rate of proliferation and colony formation compared to the same ALDH1A1low cells. Finally, overlapping results have been obtained in human breast cancer cells, in which the expression of ALDH1A1 was involved in the regulation of adhesion, migration, and colony formation and in the acquisition of metastatic phenotype. Likewise, experimental studies have demonstrated that CSCs with high ALDH activity identified by Aldefluor assay® showed high tumorigenic phenotype in vitro and in vivo. Moreover, the prognostic value of ALDH1A1 in cancers has been validated by using immunohistochemistry (IHC) on cancer patient tissues. ALDH1A1 overexpression evaluated by IHC has been correlated with poor prognosis in many cancers such as lung, ovarian, stomach, breast, and colorectal cancer. Various authors reported the prognostic value of ALDH1A1 in breast cancer patients. It seems to be associated with larger tumor size, lymph node infiltration, and poor outcome in systematic review and meta-analysis[16,62]. However, controversial data have been described in other tumors such as pancreas adenocarcinoma[63,64] and malignant melanoma[65,66]. Explaining these discrepancies is not easy, but type of cancer and degree of maturation could be reasonable causes.
The remodeling of the tumor microenvironment (TME) in which CSCs reside is related to their ability to drive tumor growth and metastases. TME is an integral part of the physiology, structure, and functions of CSCs, conditioning the fate of tumor cells in each state of differentiation. On the one hand, by providing various paracrine factors, TME plays a crucial role in maintaining CSC plasticity, by regulating pathways or transcription factors involved in self-renewal process. On the other hand, CSCs can actively recruit some of these niche components to create a microenvironment that is favorable for its survival. For example, CSCs can secrete vascular endothelial growth factor (VEGF) or other factors to recruit perivascular cells. TME consists in multiple types of cells such as endothelial cells, fibroblasts, mast cells, neutrophils, perivascular cells, adipocytes, macrophages, and immune-suppressive cells. The contribution of stromal cells in the regulation of tumor hallmarks is now recognized, and the most important role of endothelial cells on tumor growth and the expansion of self-renewing CSCs, through the neovascularisation, is well-established. An important observation by Balic et al. is the greater proportion of CSCs present in metastatic tumors compared to the primary site. The process known as “angiogenic switch” reflects increased bioavailability of growth factors for vascular cells, allowing the removal of cellular waste products. The regulation of angiogenic phenotype by CSCs in many tumors is widely reported in the literature. Bao et al. demonstrated that stem-like glioma cells (CD133+) from human glioblastoma biopsy developed tumors having extensive angiogenesis in a VEGF-dependent manner. ALDH1A1 activates retinoic acid signaling, which, in turn, regulates hypoxia inducible factor-1α and the VEGF axis[73,74]. Lv et al. obtained similar results in patients with invasive ductal breast carcinoma expressing ALDH1 enzyme. Many researchers obtained overlapping results in other tumor types. Silva et al. suggested a potential control of angiogenic ovarian cancer based on ALDH activity and CD133 expression. It has also been reported that tumor endothelial cells showed increased expression of VEGFR2 and a highly angiogenic phenotype compared to normal endothelial cells. This behavior was related to ALDH1A1 expression in the same endothelial cells. Finally, in a co-culture system of human stem cells from bone marrow, ALDH1A1high cells promote endothelial cell organization on Matrigel.
Angiogenesis is required for tumor growth and metastases since new aberrant vessels are easily permeable to highly invasive tumor cells. Metastasis represents the main problem for cancer therapy. The possibility that cancer cells can switch into CSCs under specific conditions dictated by a depletion in the number of CSCs or by environment can open a new perspective on the metastatic process. There is growing evidence supporting the role of ALDH1A1 in cancer invasive phenotype and the ability to metastasize. Its expression has been reported to correlate with increased in vitro migration in NSCLC, renal, esophageal, and breast cancer cells. Summarizing, increasing evidence from in vitro studies offers a mechanistic role for ALDH1A1 in metastases. Likewise, functional association of ALDH1A1 and in vivo metastases has been suggested. Ginestier’s group found high ALDH activity in cell lines derived from mammary tissue. The same cells with high ALDH activity (Aldefluor-positive) showed stemness phenotype and metastatic potential. This was the first observation of ALDH activity in tumor stemness and progression. Subsequent studies have shown that ALDH1A1 was involved in increased metastatic capability of prostate[81,82], breast, colon, ovarian, and esophageal cancer, suggesting that the activity of the enzyme also has a functional role in more differentiated cancer cells, as well as in CSCs[48,49]. It has also been reported that depletion of ALDH1A1 by shRNA in melanoma cells resulted in significant delay in appearance of xenografted melanoma and reduction in tumor growth, as well as a decrease of metastases number after tail vein injection in mice.
Besides cancer progression and invasion, CSCs are suggested to be responsible for drug resistance, and cancer recurrence can be due in part to their ability to renew themselves and differentiate into a heterogeneous lineage of cancer cells. The mechanisms by which CSCs escape radio- and chemotherapy include epithelial-to-mesenchymal transition, multidrug resistance, dormancy, and tumor microenvironment composition. Enhanced ALDH1A1 is a putative marker of resistance to chemotherapeutic agents, as well as immunotherapy and antiangiogenic strategies. This is not surprising because of the cellular protective role of this enzyme against various harmful aldehydes. In 1984, Hilton observed a chemoresistance role for ALDH in leukemic stem cells against cyclophosphamide, an alkylating agent. ALDH metabolizes cyclophosphamide to 4-hydroperoxycyclophosphamide, an inactive excretory product. He found a reversion of cyclophosphamide resistance by inhibiting ALDH activity with disulfiram. Subsequent studies showed that ALDH1 could confer resistance in other cancers such as breast cancer. The role of ALDH1A1 in drug metabolism has been demonstrated in a pharmacogenetic analysis. In 882 breast cancer patients treated with cyclophosphamide and doxorubicin, two SNPs of ALDH1A1 associated with an increased risk of hematological toxicity, grades 3 and 4, have been discovered. In addition to conferring resistance to cyclophosphamide, ALDH1A1 knockdown experiments demonstrated its role in pancreatic adenocarcinoma cells sensitized to the gemcitabine effect. Kulsum et al. showed that, in head and neck cancer cell lines resistant to cisplatin and 5-fluorouracil, there was an enrichment of ALDH1A1 overexpressing cells as compared to parental cells.
Recently, an ALDH1A1-induced resistance has also been found against tyrosine kinase inhibitors in in vitro and in vivo models of lung adenocarcinoma through the upregulation of superoxide dismutase and glutathione peroxidase 4 as well as ALDH1A1 itself, key enzymes involved in the redox signaling. An upregulation of ALDH1A1 expression in non-small cell lung cancer resistant to cisplatin can be a consequence of sex-determining region Y-box-9 activation. On the contrary, ALDH1A1 depletion could reverse cisplatin resistance in human lung cancer cells.
In ovarian cancer, Kaipio et al. observed that ALDH1A1 was increased after taxane and platinum treatment and its expression is correlated with chemoresistance and reduced survival. In the same tumors cells, Świerczewska et al. showed that high levels of ALDH1A1 are linked to resistance to cytotoxic agents through protein tyrosine phosphatase receptor type K (PTPRK) downregulation.
Furthermore, Mori et al. demonstrated in a model of spheroid cells derived from human endometrial cancer that ALDH1A1 inhibition synergized with paclitaxel to block cancer proliferation.
The role of ALDH1A1 in mediating radioresistance in prostate cancer cells was proposed by Cojoc et al.. They demonstrated that inhibition of Wnt pathway leads to a reduction of ALDH positive population and restores the response to radiotherapy.
Increased expression of stem cell markers correlated with enhanced stem-like features, migration, and in vivo tumor burden, demonstrating that chemoresistance, stemness, and metastases are strictly interwoven. The acquisition of a quiescent state from CSCs could be due to the selection pressure imposed by chemotherapy. It is primarily due to slow cell cycling, lower proliferation, increased expression of DNA repair, and anti-apoptosis genes.
Since most cytotoxic chemotherapeutic agents preferentially target the most proliferative cells, quiescence (or dormancy) renders CSCs insensitive to these drugs.
Recently, resistance against biological drugs through CSC involvement has also been established. Therefore, there are many clinical failures due to the development of inherent/acquired resistance to antiangiogenic drugs, but the role of ALDH1A1 in this context is debated. Various studies have found a correlation between the expression of stemness marker and resistance to antiangiogenic drugs[100-102]. A critical point is that tumor angiogenesis is not only a VEGF-dependent process since there are several key factors responsible for this phenomenon, but most drugs inhibit the VEGF signaling.
Finally, the acquisition of stem-like phenotype is a putative mechanism by which CSCs develop refractoriness to current immunotherapeutic treatment strategies[103,104]. Resistance of CSCs to immunotherapy has been reported in a glioma model of vaccination with dendritic cells. This is consistent with the ability of tumors to avoid the immune destruction. Emerging literature shows that the enrichment of PD-L1 expression in CSCs contributes to CSC immune evasion. In 2019, Castagnoli et al. showed an enrichment of PD-L1 expression in tissues from ALDH1A1 positive and triple negative breast cancer patients. Interestingly, clinical samples and 4T1 murine mammary cells (Aldefluor+) express lower levels of antigen processing and presentation proteins (TAP-1 and TAP-2) and co-stimulatory molecules (CD80). This pattern of expression decreases the susceptibility of CSCs to immune T cell-mediated attack.
In the complex, the interaction between CSCs and the immune system is of much interest, as their increased tumorigenicity suggests they may have enhanced immunoediting mechanisms.
The idea to develop therapeutic anti-cancer strategies that consider the heterogeneity of cancer is a medical option in the field of tumor biology and treatment. CSCs are an excellent example of tumor plasticity and the preclinical and clinical evidence suggests their importance in cancer progression, relapse, metastases, and chemoresistance. The discovery of phenotypic switching of tumor cells into CSCs and vice versa opens new possible therapeutic options. It has been ascertained that environment and genetic alterations regulate CSC switch. ALDH1A1 is responsible for the maintenance of the stem-like state in tumor cells, in which several hallmarks of cancer are activated [Figure 1], and it is also frequently overexpressed and/or activated in more differentiated cancer cells. The switching from undifferentiated state to differentiated state is highly dynamic and the fate of stem-like state can be decided by ALDH1A1 expression/activity in tumor cells, suggesting that this enzyme might represent a concrete novel target for cancer treatment.
Wrote the manuscript and drew the figure: Ciccone V
Made substantial contribution in the revision process: Morbidelli L, Donnini S
Gave final approval of the submitted version and revised version to be submitted: Ziche MAvailability of data and materials
Not applicable.Financial support and sponsorship
This research was partially funded by MIUR-PRIN (2017XP72RF) to Morbidelli L.Conflicts of interest
All authors declared that there are no conflicts of interest.Ethical approval and consent to participate
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© The Author(s) 2020.
1. Hanahan D, Weinberg RA. The hallmarks of cancer. Cell 2000;100:57-70.DOIPubMed
2. Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell 2011;144:646-74.DOIPubMed
3. Horne SD, Pollick SA, Heng HH. Evolutionary mechanism unifies the hallmarks of cancer. Int J Cancer 2015;136:2012-21.DOIPubMed
4. Saygin C, Matei D, Majeti R, Reizes O, Lathia JD. Targeting cancer stemness in the clinic: from hype to hope. Cell Stem Cell 2019;24:25-40.DOIPubMed
5. Schulenburg A, Blatt K, Cerny-Reiterer S, Sadovnik I, Herrmann H, et al. Cancer stem cells in basic science and in translational oncology: can we translate into clinical application? J Hematol Oncol 2015;8:16.DOIPubMedPMC
6. Skvortsova I, Debbage P, Kumar V, Skvortsov S. Radiation resistance: cancer stem cells (CSCs) and their enigmatic pro-survival signaling. Semin Cancer Biol 2015;35:39-44.DOIPubMed
7. Iscove NN, Nawa K. Hematopoietic stem cells expand during serial transplantation in vivo without apparent exhaustion. Curr Biol 1997;7:805-8.DOIPubMed
8. Yin H, Xu L, Porter NA. Free radical lipid peroxidation: mechanisms and analysis. Chem Rev 2011;111:5944-72.DOIPubMed
9. Grune T. Protein oxidation products as biomarkers. Free Radic Biol Med 2014;75 Suppl 1:S7.DOI
10. O’Brien PJ, Siraki AG, Shangari N. Aldehyde sources, metabolism, molecular toxicity mechanisms, and possible effects on human health. Crit Rev Toxicol 2005;35:609-62.DOIPubMed
11. Vassalli G. Aldehyde dehydrogenases: not just markers, but functional regulators of stem cells. Stem Cells Int 2019;2019:3904645.DOIPubMedPMC
12. Dockham PA, Sreerama L, Sladek NE. Relative contribution of human erythrocyte aldehyde dehydrogenase to the systemic detoxification of the oxazaphosphorines. Drug Metab Dispos 1997;25:1436-41.PubMed
13. Allison SE, Chen Y, Petrovic N, Zhang J, Bourget K, et al. Activation of ALDH1A1 in MDA-MB-468 breast cancer cells that over-express CYP2J2 protects against paclitaxel-dependent cell death mediated by reactive oxygen species. Biochem Pharmacol 2017;143:79-89.DOIPubMed
14. Yoshida A, Davé V, Han H, Scanlon KJ. Enhanced transcription of the cytosolic ALDH gene in cyclophosphamide resistant human carcinoma cells. Adv Exp Med Biol 1993;328:63-72.DOIPubMed
15. Notas G, Pelekanou V, Kampa M, Alexakis K, Sfakianakis S, et al. Tamoxifen induces a pluripotency signature in breast cancer cells and human tumors. Mol Oncol 2015;9:1744-59.DOIPubMedPMC
16. Khoury T, Ademuyiwa FO, Chandrasekhar R, Jabbour M, Deleo A, et al. Aldehyde dehydrogenase 1A1 expression in breast cancer is associated with stage, triple negativity, and outcome to neoadjuvant chemotherapy. Mod Pathol 2012;25:388-97.DOIPubMedPMC
17. Chen DS, Mellman I. Elements of cancer immunity and the cancer-immune set point. Nature 2017;541:321-30.DOIPubMed
18. Tomasetti C, Vogelstein B. Variation in cancer risk among tissues can be explained by the number of stem cell divisions. Science 2015;347:78-81.DOIPubMedPMC
19. Valent P, Bonnet D, De Maria R, Lapidot T, Copland M, et al. Cancer stem cell definitions and terminology: the devil is in the details. Nat Rev Cancer 2012;12:767-75.DOIPubMed
20. Batlle E, Clevers H. Cancer stem cells revisited. Nat Med 2017;23:1124-34.DOIPubMed
21. Phi LTH, Sari IN, Yang YG, Lee SH, Jun N, et al. Cancer stem cells (CSCs) in drug resistance and their therapeutic implications in cancer treatment. Stem Cells Int 2018;2018:5416923.DOIPubMedPMC
22. Chen W, Dong J, Haiech J, Kilhoffer MC, Zeniou M. Cancer stem cell quiescence and plasticity as major challenges in cancer therapy. Stem Cells Int 2016;2016:1740936.DOIPubMedPMC
23. Annett S, Robson T. Targeting cancer stem cells in the clinic: current status and perspectives. PharmacolTher 2018;187:13-30.DOIPubMed
24. Zhao W, Li Y, Zhang X. Stemness-related markers in cancer. Cancer Transl Med 2017;3:87-95.DOIPubMedPMC
25. Huang EH, Hynes MJ, Zhang T, Ginestier C, Dontu G, et al. Aldehyde dehydrogenase 1 is a marker for normal and malignant human colonic stem cells (SC) and tracks SC overpopulation during colon tumorigenesis. Cancer Res 2009;69:3382-9.DOIPubMedPMC
26. Ginestier C, Hur MH, Charafe-Jauffret E, Monville F, Dutcher J, et al. ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome. Cell Stem Cell 2007;1:555-67.DOIPubMedPMC
27. Jiang F, Qiu Q, Khanna A, Todd NW, Deepak J, et al. Aldehyde dehydrogenase 1 is a tumor stem cell-associated marker in lung cancer. Mol Cancer Res 2009;7:330-8.DOIPubMedPMC
28. Li T, Su Y, Mei Y, Leng Q, Leng B, et al. ALDH1A1 is a marker for malignant prostate stem cells and predictor of prostate cancer patients’ outcome. Lab Invest 2010;90:234-44.DOIPubMedPMC
29. Li Y, Lin K, Yang Z, Han N, Quan X, et al. Bladder cancer stem cells: clonal origin and therapeutic perspectives. Oncotarget 2017;8:66668-79.DOIPubMedPMC
30. Landen CN, Goodman B, Katre AA, Steg AD, Nick AM, et al. Targeting aldehyde dehydrogenase cancer stem cells in ovarian cancer. Mol Cancer Ther 2010;9:3186-99.DOIPubMedPMC
31. Moreb JS. Aldehyde dehydrogenase as a marker for stem cells. Curr Stem Cell Res Ther 2008;3:237-46.DOIPubMed
32. Sullivan JP, Spinola M, Dodge M, Raso MG, Behrens C, et al. Aldehyde dehydrogenase activity selects for lung adenocarcinoma stem cells dependent on notch signaling. Cancer Res 2010;70:9937-48.DOIPubMedPMC
33. Minato T, Yamamoto Y, Seike J, Yoshida T, Yamai H, et al. Aldehyde dehydrogenase 1 expression is associated with poor prognosis in patients with esophageal squamous cell carcinoma. Ann Surg Oncol 2013;20:209-17.DOIPubMed
34. Li X, Xu Q, Fu X, Luo W. ALDH1A1 overexpression is associated with the progression and prognosis in gastric cancer. BMC Cancer 2014;14:705.DOIPubMedPMC
35. Somasagara RR, Spencer SM, Tripathi K, Clark DW, Mani C, et al. RAD6 promotes DNA repair and stem cell signaling in ovarian cancer and is a promising therapeutic target to prevent and treat acquired chemoresistance. Oncogene 2017;36:6680-90.DOIPubMedPMC
36. Xu SL, Zeng DZ, Dong WG, Ding YQ, Rao J, et al. Distinct patterns of ALDH1A1 expression predict metastases and poor outcome of colorectal carcinoma. Int J Clin Exp Pathol 2014;7:2976-86.PubMedPMC
37. Seitz HK, Stickel F. Molecular mechanisms of alcohol-mediated carcinogenesis. Nat Rev Cancer 2007;7:599-612.DOIPubMed
38. Niederreither K, Subbarayan V, Dollé P, Chambon P. Embryonic retinoic acid synthesis is essential for early mouse post-implantation development. Nature Genetics 1999;21:444-8.DOIPubMed
39. Marcato P, Dean CA, Liu RZ, Coyle KM, Bydoun M, et al. Aldehyde dehydrogenase 1A3 influences breast cancer progression via differential retinoic acid signaling. Mol Oncol 2015;9:17-31.DOIPubMedPMC
40. Luo Y, Dallaglio K, Chen Y, Robinson WA, Robinson SE, et al. ALDH1A isozymes are markers of human melanoma stem cells and potential therapeutic targets. Stem Cells 2012;30:2100-13.DOIPubMedPMC
41. Xu X, Chai S, Wang P, Zhang C, Yang Y, et al. Aldehyde dehydrogenases and cancer stem cells. Cancer Lett 2015;369:50-7.DOIPubMed
42. Marchitti SA, Brocker C, Stagos D, Vasiliou V. Non-P450 aldehyde oxidizing enzymes: the aldehyde dehydrogenase superfamily. Expert Opin Drug MetabToxicol 2008;4:697-720.DOIPubMedPMC
43. Celià-Terrassa T, Kang Y. Metastatic niche functions and therapeutic opportunities. Nat Cell Biol 2018;20:868.DOIPubMed
44. Aponte PM, Caicedo A. Stemness in cancer: stem cells, cancer stem cells, and their microenvironment. Stem Cells Int 2017;2017:5619472.DOIPubMedPMC
45. Plaks V, Kong N, Werb Z. The cancer stem cell niche: how essential is the niche in regulating stemness of tumor cells? Cell Stem Cell 2015;16:225-38.DOIPubMedPMC
46. Visvader JE, Clevers H. Tissue-specific designs of stem cell hierarchies. Nat Cell Biol 2016;18:349-55.DOIPubMed
47. Menendez JA, Alarcón T. Metabostemness: a new cancer hallmark. Front Oncol 2014;4:262.DOIPubMedPMC
48. Reya T, Morrison SJ, Clarke MF, Weissman IL. Stem cells, cancer, and cancer stem cells. Nature 2001;414:105-11.DOIPubMed
49. Beck B, Blanpain C. Unravelling cancer stem cell potential. Nat Rev Cancer 2013;13:727-38.DOIPubMed
50. La Porta CAM, Zapperi S. Complexity in cancer stem cells and tumor evolution: toward precision medicine. Semin Cancer Biol 2017;44:3-9.DOIPubMed
51. Tomita H, Tanaka K, Tanaka T, Hara A. Aldehyde dehydrogenase 1A1 in stem cells and cancer. Oncotarget 2016;7:11018-32.DOIPubMedPMC
52. Johnson S, Chen H, Lo PK. In vitro tumorsphere formation assays. Bio Protoc 2013;3:e325.PubMedPMC
53. Friedl P, Alexander S. Cancer invasion and the microenvironment: plasticity and reciprocity. Cell 2011;147:992-1009.DOIPubMed
54. Lu P, Takai K, Weaver VM, Werb Z. Extracellular matrix degradation and remodeling in development and disease. Cold Spring Harb Perspect Biol 2011;3:a005058.DOIPubMedPMC
55. Chen Y, Orlicky DJ, Matsumoto A, Singh S, Thompson DC, et al. Aldehyde dehydrogenase 1B1 (ALDH1B1) is a potential biomarker for human colon cancer. Biochem Biophys Res Commun 2011;405:173-9.DOIPubMedPMC
56. Marchitti SA, Orlicky DJ, Brocker C, Vasiliou V. Aldehyde dehydrogenase 3B1 (ALDH3B1): immunohistochemical tissue distribution and cellular-specific localization in normal and cancerous human tissues. J Histochem Cytochem 2010;58:765-83.DOIPubMedPMC
57. Patel M, Lu L, Zander DS, Sreerama L, Coco D, et al. ALDH1A1 and ALDH3A1 expression in lung cancers: correlation with histologic type and potential precursors. Lung Cancer 2008;59:340-9.DOIPubMed
58. Leid M, Kastner P, Chambon P. Multiplicity generates diversity in the retinoic acid signalling pathways. Trends Biochem Sci 1992;17:427-33.DOIPubMed
59. Yang L, Ren Y, Yu X, Qian F, Bian BS, et al. ALDH1A1 defines invasive cancer stem-like cells and predicts poor prognosis in patients with esophageal squamous cell carcinoma. Modern Pathology 2014;27:775-83.DOIPubMed
60. Xu SL, Liu S, Cui W, Shi Y, Liu Q, et al. Aldehyde dehydrogenase 1A1 circumscribes high invasive glioma cells and predicts poor prognosis. Am J Cancer Res 2015;5:1471-83.PubMedPMC
61. Croker AK, Rodriguez-Torres M, Xia Y, Pardhan S, Leong HS, et al. Differential functional roles of ALDH1A1 and ALDH1A3 in mediating metastatic behavior and therapy resistance of human breast cancer cells. Int J Mol Sci 2017;18:E2039.DOIPubMedPMC
62. Liu Y, Lv D, Duan J, Xu SL, Zhang JF, et al. ALDH1A1 expression correlates with clinicopathologic features and poor prognosis of breast cancer patients: a systematic review and meta-analysis. BMC Cancer 2014;14:444.DOIPubMedPMC
63. Kahlert C, Bergmann F, Beck J, Welsch T, Mogler C, et al. Low expression of aldehyde dehydrogenase 1A1 (ALDH1A1) is a prognostic marker for poor survival in pancreatic cancer. BMC Cancer 2011;11:275.DOIPubMedPMC
64. Rasheed ZA, Yang J, Wang Q, Kowalski J, Freed I, et al. Prognostic significance of tumorigenic cells with mesenchymal features in pancreatic adenocarcinoma. J Natl Cancer Inst 2010;102:340-51.DOIPubMedPMC
65. Prasmickaite L, Engesaeter BØ, Skrbo N, Hellenes T, Kristian A, et al. Aldehyde dehydrogenase (ALDH) activity does not select for cells with enhanced aggressive properties in malignant melanoma. PLoS One 2010;5:e10731.DOIPubMedPMC
66. Boonyaratanakornkit JB, Yue L, Strachan LR, Scalapino KJ, LeBoit PE, et al. Selection of tumorigenic melanoma cells using ALDH. J Invest Dermatol 2010;130:2799-808.DOIPubMedPMC
67. Ping YF, Zhang X, Bian XW. Cancer stem cells and their vascular niche: do they benefit from each other? Cancer Lett 2016;380:561-7.DOIPubMed
68. Hanahan D, Coussens LM. Accessories to the crime: functions of cells recruited to the tumor microenvironment. Cancer Cell 2012;21:309-22.DOIPubMed
69. Salmaggi A, Boiardi A, Gelati M, Russo A, Calatozzolo C, et al. Glioblastoma-derived tumorospheres identify a population of tumor stem-like cells with angiogenic potential and enhanced multidrug resistance phenotype. Glia 2006;54:850-60.DOIPubMed
70. Balic M, Lin H, Young L, Hawes D, Giuliano A, et al. Most early disseminated cancer cells detected in bone marrow of breast cancer patients have a putative breast cancer stem cell phenotype. Clin Cancer Res 2006;12:5615-21.DOIPubMed
71. Bickel ST, Juliano JD, Nagy JD. Evolution of proliferation and the angiogenic switch in tumors with high clonal diversity. PLoS One 2014;9:e91992.DOIPubMedPMC
72. Bao S, Wu Q, Sathornsumetee S, Hao Y, Li Z, et al. Stem cell-like glioma cells promote tumor angiogenesis through vascular endothelial growth factor. Cancer Res 2006;66:7843-8.DOIPubMed
73. Ciccone V, Terzuoli E, Donnini S, Giachetti A, Morbidelli L, et al. Stemness marker ALDH1A1 promotes tumor angiogenesis via retinoic acid/HIF-1α/VEGF signalling in MCF-7 breast cancer cells. J Exp Clin Cancer Res 2018;37:311.DOIPubMedPMC
74. Ciccone V, Terzuoli E, Donnini S, Giachetti A, Morbidelli L, et al. Correction to: stemness marker ALDH1A1 promotes tumor angiogenesis via retinoic acid/HIF-1α/VEGF signalling in MCF-7 breast cancer cells. J Exp Clin Cancer Res 2019;38:45.DOIPubMedPMC
75. Lv X, Wang Y, Song Y, Pang X, Li H. Association between ALDH1+/CD133+ stem-like cells and tumor angiogenesis in invasive ductal breast carcinoma. Oncol Lett 2016;11:1750-6.DOIPubMedPMC
76. Silva IA, Bai S, McLean K, Yang K, Griffith K, et al. Aldehyde dehydrogenase in combination with CD133 defines angiogenic ovarian cancer stem cells that portend poor patient survival. Cancer Res 2011;71:3991-4001.DOIPubMedPMC
77. Ohmura-Kakutani H, Akiyama K, Maishi N, Ohga Noritaka, Hida Y, et al. Identification of tumor endothelial cells with high aldehyde dehydrogenase activity and a highly angiogenic phenotype. PLoS One 2014;9:e113910.DOIPubMedPMC
78. White HS, Smith L, Gentry T, Balber AE. Mechanisms of action of human aldehyde dehydrogenase bright cells in therapy of cardiovascular diseases: expression analysis of angiogenic factors and aldehyde dehydrogenase isozymes. J Stem Cell Res Ther 2011; doi: 10.4172/2157-7633.S1-001.DOI
79. Li X, Wan L, Geng J, Wu CL, Bai X. Aldehyde dehydrogenase 1A1 possesses stem-like properties and predicts lung cancer patient outcome. J Thorac Oncol 2012;7:1235-45.DOIPubMed
80. Wang K, Chen X, Zhan Y, Jiang W, Liu X, et al. Increased expression of ALDH1A1 protein is associated with poor prognosis in clear cell renal cell carcinoma. Med Oncol 2013;30:574.DOIPubMed
81. Van den Hoogen C, van der Horst G, Cheung H, Buijs JT, Lippitt JM, et al. High aldehyde dehydrogenase activity identifies tumor-initiating and metastases-initiating cells in human prostate cancer. Cancer Res 2010;70:5163-73.DOIPubMed
82. Kalantari E, Saadi FH, Asgari M, Shariftabrizi A, Roudi R, et al. Increased expression of ALDH1A1 in prostate cancer is correlated with tumor aggressiveness: a tissue microarray study of iranian patients. Appl Immunohistochem Mol Morphol 2017;25:592-8.DOIPubMed
83. Yao J, Jin Q, Wang XD, Zhu HJ, Ni QC. Aldehyde dehydrogenase 1 expression is correlated with poor prognosis in breast cancer. Medicine (Baltimore) 2017;96:e7171.DOIPubMedPMC
84. van der Waals LM, Rinkes IHMB, Kranenburg O. ALDH1A1 expression is associated with poor differentiation, ‘right-sidedness’ and poor survival in human colorectal cancer. PLoS One 2018;13:e0205536.DOIPubMedPMC
85. Liao J, Qian F, Tchabo N, Mhawech-Fauceglia P, Beck A, et al. Ovarian cancer spheroid cells with stem cell-like properties contribute to tumor generation, metastases and chemotherapy resistance through hypoxia-resistant metabolism. PLoS One 2014;9:e84941.DOIPubMedPMC
86. Sun L, Cao J, Guo C, Burnett J, Yang Z, et al. Associations of carboxypeptidase 4 with ALDH1A1 expression and their prognostic value in esophageal squamous cell carcinoma. Dis Esophagus 2017;30:1-5.DOIPubMed
87. Yue L, Huang ZM, Fong S, Leong S, Jakowatz JG, et al. Targeting ALDH1 to decrease tumorigenicity, growth and metastases of human melanoma. Melanoma Res 2015;25:138-48.DOIPubMed
88. Hilton J. Role of aldehyde dehydrogenase in cyclophosphamide-resistant L1210 leukemia. Cancer Res 1984;44:5156-60.PubMed
89. Sreerama L, Sladek NE. Cellular levels of class 1 and class 3 aldehyde dehydrogenases and certain other drug-metabolizing enzymes in human breast malignancies. Clin Cancer Res 1997;3:1901-14.PubMed
90. Yao S, Sucheston LE, Zhao H, Barlow WE, Zirpoli G, et al. Germline genetic variants in ABCB1, ABCC1, and ALDH1A1 and risk of hematological and gastrointestinal toxicities in a SWOG Phase III trial S0221 for breast cancer. Pharmacogenomics J 2014;14:241-7.DOIPubMedPMC
91. Duong HQ, Hwang JS, Kim HJ, Kang HJ, Seong YS, et al. Aldehyde dehydrogenase 1A1 confers intrinsic and acquired resistance to gemcitabine in human pancreatic adenocarcinoma MIA PaCa-2 cells. Int J Oncol 2012;41:855-61.DOIPubMedPMC
92. Kulsum S, Sudheendra HV, Pandian R, Ravindra DR, Siddappa G, et al. Cancer stem cell mediated acquired chemoresistance in head and neck cancer can be abrogated by aldehyde dehydrogenase 1 A1 inhibition. Mol Carcinog 2017;56:694-711.DOIPubMed
93. Lei HM, Zhang KR, Wang CH, Wang Y, Zhuang GL, et al. Aldehyde dehydrogenase 1A1 confers erlotinib resistance via facilitating the reactive oxygen species-reactive carbonyl species metabolic pathway in lung adenocarcinomas. Theranostics 2019;9:7122-39.DOIPubMedPMC
94. Voronkova MA, Rojanasakul LW, Kiratipaiboon C, Rojanasakul Y. SOX9-ALDH axis determines resistance to chemotherapy in non-small cell lung cancer. Mol Cell Biol 2019; doi: 10.1128/MCB.00307-19.DOIPubMed
95. Wei Y, Wu S, Xu W, Liang Y, Li Y, et al. Depleted aldehyde dehydrogenase 1A1 (ALDH1A1) reverses cisplatin resistance of human lung adenocarcinoma cell A549/DDP. Thorac Cancer 2017;8:26-32.DOIPubMedPMC
96. Kaipio K, Chen P, Roering P, Huhtinen K, Mikkonen P, et al. ALDH1A1-related stemness in high-grade serous ovarian cancer is a negative prognostic indicator but potentially targetable by EGFR/mTOR-PI3K/aurora kinase inhibitors. J Pathol 2019; doi: 10.1002/path.5356.DOIPubMed
97. Świerczewska M, Sterzyńska K, Wojtowicz K, Kaźmierczak D, Iżycki D, et al. PTPRK expression is downregulated in drug resistant ovarian cancer cell lines, and especially in ALDH1A1 positive CSCs-like populations. Int J Mol Sci 2019;20:2053.DOIPubMedPMC
98. Mori Y, Yamawaki K, Ishiguro T, Yoshihara K, Ueda H, et al. ALDH-dependent glycolytic activation mediates stemness and paclitaxel resistance in patient-derived spheroid models of uterine endometrial cancer. Stem Cell Reports 2019;13:730-46.DOIPubMedPMC
99. Cojoc M, Peitzsch C, Kurth I, Trautmann F, Kunz-Schughart LA, et al. Aldehyde Dehydrogenase is regulated by β-catenin/TCF and promotes radioresistance in prostate cancer progenitor cells. Cancer Res 2015;75:1482-94.DOIPubMed
100. Mitsuhashi A, Goto H, Saijo A, Trung VT, Aono Y, et al. Fibrocyte-like cells mediate acquired resistance to anti-angiogenic therapy with bevacizumab. Nat Commun 2015;6:8792.DOIPubMedPMC
101. Yang H, Li X, Zhou Y, Ban X, Zeng T, et al. Stemness and chemotherapeutic drug resistance induced by EIF5A2 overexpression in esophageal squamous cell carcinoma. Oncotarget 2015;6:26079-89.DOIPubMedPMC
102. Najafi M, Mortezaee K, Majidpoor J. Cancer stem cell (CSC) resistance drivers. Life Sci 2019;234:116781.DOIPubMed
103. Schatton T, Frank MH. Antitumor immunity and cancer stem cells. Ann N Y Acad Sci 2009;1176:154-69.DOIPubMedPMC
104. In: Maccalli C, Todaro M, Ferrone S, editors. Cancer stem cell resistance to targeted therapy. Springer International Publishing; 2019.
105. Irvin DK, Jouanneau E, Duvall G, Xiao-Xue Z, Yuying Z, et al. T cells enhance stem-like properties and conditional malignancy in gliomas. PLoS One 2010;5:e10974.DOIPubMedPMC
106. Hsu JM, Xia W, Hsu YH, Chan LC, Yu WH, et al. STT3-dependent PD-L1 accumulation on cancer stem cells promotes immune evasion. Nat Commun 2018;9:1908.DOIPubMedPMC
107. Castagnoli L, Cancila V, Cordoba-Romero SL, Faraci S, Talarico G, et al. WNT signaling modulates PD-L1 expression in the stem cell compartment of triple-negative breast cancer. Oncogene 2019;38:4047-60.DOIPubMedPMC
108. Sultan M, Vidovic D, Paine AS, Huynh TT, Coyle KM, et al. Epigenetic silencing of TAP1 in Aldefluor+ breast cancer stem cells contributes to their enhanced immune evasion. Stem Cells 2018;36:641-54.DOIPubMed
109. Desai A, Yan Y, Gerson SL. Concise reviews: cancer stem cell targeted therapies: toward clinical success. Stem Cells Transl Med 2019;8:75-81.DOIPubMedPMC
Ciccone V, Morbidelli L, Ziche M, Donnini S. How to conjugate the stemness marker ALDH1A1 with tumor angiogenesis, progression, and drug resistance. Cancer Drug Resist 2020;3:26-37. http://dx.doi.org/10.20517/cdr.2019.70
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